以大肠杆菌的强启动子Ptrc和谷氨酸棒杆菌ATCC 13032的内源强启动子Pgro作为大肠杆菌的木糖异构酶基因xylA在谷氨酸棒杆菌中表达的转录起始元件,通过实验表明谷氨酸棒杆菌自身的强启动子Pgro能有效促使外源基因xylA在谷氨酸棒杆菌ATCC 13032中的表达,同时,核糖体结合位点RBS序列的优化对目的基因的表达水平有一定的影响。
The exogenous gene xylA fromE. coli MG1655 was expressed inCorynebacterium glutamicumATCC 13032 with the strong promoterPtrc of E. coli and the endogenous promoterPgro ofCorynebacterium glutamicum as transcription start components separately. The experiment result shows thatPgrocan be effective in the starting expression xylA inCorynebacterium glutamicum ATCC 13032. In addition, the sequence of RBS also has an impact on the gene expression level.