中文摘要：

对Hypocreasp．W63的β-葡萄糖苷酶酶学性质研究表明，该酶液体发酵最高酶活高达482．1U／mL，最适反应pH值为4．8，最适反应温度为65℃；乙醇体积分数为10％时对酶活有最大促进效果，乙醇耐受能力高达30％；将该酶应用于同步糖化发酵研究中，发现发酵至120h乙醇质量浓度可高达41．25g/L，与对照相比，乙醇产量均提高近2倍。该菌株所产的B一葡萄糖苷酶较高的酶活力、耐热性能、乙醇耐受性和同步糖化发酵促进效果，预示着该菌在纤维素乙醇产业化应用中具有广阔的应用前景。

英文摘要：

Studies of β -glucosidase enzymatic properties from Hypocrea sp. W63 show that, enzyme ac- tivity determined by pNPG （4 - nitrophenyl β - D - glucopyranoside） can attain to 482. 1 U/mL. The optimum reaction pH value and temperature are 4. 8 and 65 , respectively. 10% of ethanol has the greatest promotion, the β-glucosidase activity can nearly increased double and the ethanol tolerance of β -glucosidase can attain to 30%. When simultaneous saccharification and fermentation （SSF） carried out for 120 h with β - glucosidase adjunction, 41.25 g/L of ethanol can be obtained. Compared with control, the ethanol production can increase nearly 2 - fold. The β - glucosidase with high enzyme activi- ty, thermostable and alcohol tolerance for SSF application, indicate that the β - glucosidase producing strain has great potent application on cellulosic ethanol production.