中文摘要：

为研究不同浓度葛根素对热应激诱导的LLC-PK1细胞HSP70 mRNA和蛋白表达的影响,筛选出葛根素诱导HSP70表达的最佳浓度。选取处于对数增长期的猪肾小管上皮细胞,分为37℃对照组和42℃热应激组,热应激组细胞先在加入不同浓度（0.01～100μmol/L不等）葛根素的培养基中培养,热应激之后,提取总RNA和总蛋白,用RT-PCR和Western Blot检测HSP70 mRNA和蛋白的相对表达量。结果显示,42℃热应激和不同浓度的葛根素均能显著诱导LLC-PK1细胞的HSP70 mRNA和蛋白的表达,并且,随葛根素浓度升高,HSP70表达量增加,在各浓度组中,10μmol/L的葛根素诱导HSP70的表达最显著（P〈0.01）。上述结果证明,葛根素能够诱导热应激条件下LLC-PK1细胞HSP70的表达,并且随葛根素浓度升高,HSP70表达量增加。

英文摘要：

This experiment was to study the effect of puerarin on the thermal stress induced HSP70 expression in pig renal tubular epithelial cell（ LLC-PK1）,and to selected the optimal concentration of puerarin for inducing HSP70 expression.The LLC-PK1 in its logarithmic growth period was selected and divided into 37 ℃ control group and 42 ℃ heat stress group.The heat stress group of cell was incubated with various concentrations（ from 0.01-100 μmol /L） of puerarin before the heat stress treatment,and then the total RNA and protein of the cells were extracted and were detected by RT-PCR and Western Blot,respectively.The results showed that both thermal stress at42 ℃ and puerarin with various concentrations significantly induced the HSP70 expression of LLC-PK1（ P〈 0.01）,and HSP70 expression increased as the puerarin concentration increased in the culture medium.The relative expression of HSP70 in the heat stress group was the highest in the 10 μmol /L of puerarin group.The above results indicate that puerarin can induce HSP70 expression of LLC-PK1 under heat stress and this expressions increased as the puerarin concentration increased in the culture medium.