中文摘要：

为了探讨不同浓度黄芩苷对热应激条件下猪肾小管上皮（LLC-PKl）细胞HSP70表达的影响，筛选出黄芩苷诱导热应激条件下细胞HSP70表达的最佳浓度。将培养的LLC-PKI细胞随机分为7个组，I组为37℃常温对照组，Ⅱ组为42℃单纯热应激组，Ⅲ、Ⅳ、V、Ⅵ组和Ⅶ组分别为42℃热应激并用不同浓度黄芩苷（0．01～100Ixg／mL）处理组，运用实时荧光定量PCR和WesternBlot检测HSP70mRNA及蛋白的表达。结果显示，42℃单纯热应激能显著诱导LLC-PKl细胞HSP70的表达；一定浓度范围内的黄芩苷（0．1～10μg／mL）处理的LLC-PKl细胞HSP70表达显著高于42℃单纯热应激；黄芩苷（0．01～100μg／mL）能显著上调热应激条件下LLC-PKl细胞HSP70的表达，且lμg／mL的黄芩苷上调效果最显著。

英文摘要：

The objective of the experiment was to explore if the baicalin of different concentrations contributed to HSP70 ex- pression of heat-stressed LLC-PK1 cells and to select the optimal concentration of baicalin for inducing HSPT0 expression. The readily cultured LLC-PKI ceils were divided into seven groups in a random fashion. Cells in group I were served as the control and cultured at 37 ℃ cells in group Ⅱ were heat-stressed at 42 ℃ for 1 h. Group Ⅲ, IV, V, VI and VII were cultured with the addition of different concentrations of baiealin （varying from 0.01 p~g/mL to 100 p~g/mL）at 42 ℃ for 1 h, respectively. The HSP70 mRNA and protein expression were determined by using real-time PCR and western Blot. The experimental results revealed that heat stress at 42 ℃ significantly induce the HSPT0 expression of LLC-PK1 cells. HSP expression of LLC-PK1 cells treated with ba- icalin within certain concentrations （0.1 μg/mL to 10 μg/mL）was significantly elevated than that of the heat-stressed group Ⅱ at 42 ℃.Baicalin（0.01 μg/mL to 100 μg/mL） significantly enhanced HSP70 expression in the cells subjected to heat stress and addi- tion of 1 μg/mL baicalin showed the best effect.