中文摘要：

黄芩苷（baicalin,bai）是一种黄酮类化合物,能促进移植后体内胎儿的生长发育,且对胎儿发育没有毒性。为了研究黄芩苷对体外培养小鼠（Mus musculus）胚胎发育的影响及其作用机制。本研究将体外培养的小鼠胚胎随机分成对照组（CZB培养液）和实验组（4μg/mL黄芩苷处理）,采用体视显微镜分别观察胚胎各阶段发育率;采用地衣红染色法计数囊胚细胞数;实时荧光定量检测间隙连接蛋白（gap junction protein alpha-1,GJA1）、热休克蛋白70（heat shock 70,Hsp70）、B细胞淋巴瘤/白血-2蛋白（B-cellymphoma/leukemia-2,Bcl-2）和Bcl-2相关X蛋白（Bcl-2 associated X protein,Bax）的基因表达。结果显示,黄芩苷能显著提高2-细胞和4-细胞卵裂率、桑椹胚、早期囊胚、扩张囊胚发育率和囊胚孵化率（P〈0.05）,增多扩张和孵化囊胚细胞数（P〈0.05）,显著上调GJA1,下调Hsp70和Bax m RNA的表达（P〈0.05）,降低Bax/Bcl-2的比值（P〈0.05）。上述结果表明,黄芩苷通过上调GJA1基因表达、下调Hsp70和Bax基因的表达,提高体外培养小鼠囊胚发育率和囊胚质量。本研究从分子水平揭示了黄芩苷促进胚胎发育的可能作用机理,为深入研究黄芩苷的安胎作用提供基础资料。

英文摘要：

Baicalin is a kind of flavonoid, can promotes posttransplant fetal growth and development, and hasn＇t toxicity to the fetus development. The objectives of the present study were to investigate the effects of baicalin on the development of mouse（Mus musculus） embryos in vitro and it＇s possible molecular mechanism.Mouse embryos cultured in vitro were randomly divided into control group（CZB cultural medium） and experimental group（supplemented with 4 μg/mL of baicalin）. The development rates of embryos in their different stages were recorded, respectively, using a stereoscopic microscope, and also the nuclei of blastocysts were stained with orcein for cell number counting. The expressions of gap junction protein alpha-1（GJA1）, heat Shock 70（Hsp70）, B- cellymphoma/leukemia- 2（Bcl- 2） and Bcl- 2 associated X protein（Bax）m RNA were detected by using qRT-PCR. The results showed that baicalin treatment, as compared to control group, significantly improved（P〈0.05） 2- cell and 4- cell embryonic cleavage rates, morula, blastocyst and expanded blastocyst development rates and blastocyst hatching rates, and also significantly（P〈0.05）improved the cell number of expanded and hatched blastocysts. In addition, the expression of GJA1 m RNA was significantly increased（P〈0.05）, and the expressions of Hsp70 and Bax m RNA were significantly decreased（P〈0.05）, and also the ratio between Bax/Bcl-2 was significantly reduced（P〈0.05）. We concluded that baicalin improves the blastocyst development rates and the quality of blastocysts of mouse embryo in vitro, probably, by upregulating GJA1 m RNA expression and downregulating Hsp70 and Bax m RNA expressions. The study reveals the possible mechanism of action that the baicalin promotes embryonic development, and provides basic data for the study of baicalin tocolysis role.