中文摘要：

目的 制备靶向人肝细胞肝癌表面抗原Hab18g的MR特异性抗体显影剂Hab18-SPIO，探讨其合成原理、制备要点及物理性状。方法 利用化学交联方法，采用人肝细胞癌单抗Hab18标记SPIO，制作出靶向显影剂Hab18-SPIO。并用同样方法制备阴性抗体显影剂SED-SPIO（SED为葡萄球菌肠毒素D抗体）。使用电镜及高压液相色谱仪（HPLC）对Hab18-SPIO进行测试。结果 经电镜观察和液相色谱分析证踢Hab18与SPIO有效地结合在一起，通过蛋白质含量测定得到抗体与SPIO的交联率为97．5％。经电镜观察制备的靶向显影剂Hab18-SPIO粒径在50-70nm之间，并且SPIO中颗粒较小的部分与抗体的交联较多。结论 成功构建了靶向人肝细胞肝癌表面抗原Habl8g的探针，该探针具有良好的理化性质，为进一步研究早期肝癌特异性MR诊断提供了有价值的手段。

英文摘要：

Objective To prepare the Target - directed probe （ Hab18 - SPIO ） and to test its phisichemical characteristics. Methods The MeAb Target - directed probe （ Hab18 - SPIO ） was prepared through hepatoeellular carcinoma （ HCC ） MeAb （ Hab18 ） conjugated to SPIO using chemical method , while negative MeAb probe （ SED - SPIO ） was also prepared using the same method . These agents were tested by high - performance liquid chromatography （ HPLC ） and electron microscope . Results The results of HPLC and electron microscope proved that Hab18 was conjugated SPIO effectively. The diameter of particles was between 50 and 70 nm. The binding rate of Hab18 was 97.5%. Conclusion The probe targeted to membrane - associated antigen of HCC （ Hab18g） has been successfully prepared , which has novel physiochemical property. It is of great value in specific MR diagnosis of early HCCs.