中文摘要：

目的研究膜相关抗原HAb18抗原基因（HAb18G）在荷人肝细胞肝癌裸鼠模型中的表达，探讨其在分子成像中的应用价值。方法将对数生长期人肝细胞肝癌FHCC-98制成细胞悬液（1×10^7／m1），取0．2m1分别注射于16只裸鼠胁部皮下（1处／只），建立裸鼠肝癌动物模型。对照组（3只）裸鼠仅注射无血清RPMI-1640培养液。应用MR平扫及增强扫描观察其生长。扫描后处死裸鼠，取肿瘤标本，漂洗、固定、石蜡连续切片，应用免疫组织化学方法从蛋白质水平观察膜相关抗原HAb18G在该模型上的表达。结果应用该方法建立动物模型的成瘤率高（16／16），肿瘤生长良好。对照组无肿瘤生长。MRI主要表现为长T1、长T2信号。膜相关抗原HAb18G免疫组织化学染色阳性表现为胞膜与胞质内出现棕黄色颗粒。在16只荷人肝细胞肝癌裸鼠模型中均呈强阳性表达，而对照组裸鼠肝组织则无HAb18G表达（P〈0．01）。结论荷人肝细胞肝癌裸鼠模型高表达膜相关抗原HAb18G，是基于HAbl8G为靶的活体分子成像研究的理想动物模型。

英文摘要：

Objective To investigate the expression and significance of HAb18G in a nude mouse model with human hepatoeellular carcinoma （HCC）. Methods FHCC-98, a human HCC cell line, was implanted subcutaneously to the costal regions of 16 nude mice by injection （1 ×10^7/ml, 0. 2 ml, respectively）. The culture fluid, RPMI-1640, without HCC cell line was injected in the nude mice of control group. Pre- and post-contrast enhanced MR imaging were used to observe the tumor growth. After all imaging studies, the tumor specimens were taken out for immunohistoehemical staining to observe the expression of HAb18G at protein level. Results All the tumors grew well, and the rate of establishment was high （ 16/ 16） using subcutaneous implantation as described in this paper. No tumor could be found in the nude mice of control group. The tumors showed hypointense on T1 WI and hyperintense on T2WI, and they enhanced moderately to slightly on contrast enhanced MRI. All 16 mice demonstrated strong positive expression of HAb18G, while the control group were found to be negative （P 〈 0. 01 ）. The pathological findings were similar to those in human HCC. Conclusion This HCC nude mouse model highly expressed the membraneassociated antigen HAb18G, and it was a suitable model for in vivo molecular targeting research of HAb18G.