中文摘要：

目的观察重组人内抑素（rhEndostatin）对佐剂性关节炎（AA）大鼠滑膜组织p21,细胞周期素D1（cyclin D1）及细胞周期素依赖性激酶4（CDK4）基因表达的影响,探讨rhEndostatin抑制AA大鼠成纤维样滑膜细胞（FLS）增殖的分子机制。方法雄性SD大鼠36只,随机分为正常组（n=12）、AA模型组（n=12）和rhEndostatin（2.5mg/kg）治疗组（n=12）。制备AA大鼠模型,分别采用实时荧光定量PCR和Western blotting方法,定量分析rhEndostatin对AA大鼠滑膜组织p21、cyclin D1、CDK4 mRNA及cyclin D1蛋白表达的影响。结果rhEndostatin治疗组大鼠滑膜组织p21、cyclin D1 mRNA和cyclin D1蛋白表达水平降低,CDK4 mRNA表达水平增加,与AA模型组比较,差异均有统计学意义（P〈0.01）。结论rhEndostatin降低AA大鼠滑膜组织cyclin D1表达水平可能是其抑制AA FLS增殖的分子机制之一。

英文摘要：

Objective To observe the effect of recombinant human endostatin（rhEndostatin） on the expression of p21, cyclin D1 and CDK4 genes in synovial tissue in rats with adjuvant arthritis（AA） and explore molecular mechanisms of the inhibitory effect of rhEndostatin on proliferation of fibroblast-like synoviocytes（FLS） in AA rats. Methods Thirty-six male SD rats were used in the study. They were divided at random into normal, AA model and rhEndostatin （2.5mg/kg）-treated groups. Twelve rats were taken from each group. Adjuvant arthritis was induced in rats in AA model and rhEndostatin（2.5mg/kg）-treated group. The effect of rhEndostatin on the expression of p21, eyelin D1, CDK4 mRNA and cyelin D1 protein in synovial tissue in AA rats was examined quantitatively by real-time fluorescent quantitative PCR and Westem blotting assays, respectively. Results The expression levels of p21, cyelinD1 mRNA and eyelinD1 protein were significantly decreased by rhEudostatin, compared with the levels in AA synovial tissues （P 〈 0.01 ）. However, CDK4 mRNA expression levels in synovial tissue treated with rhEndostatin were significantly higher than those in AA control samples （ P 〈 0.01 ）. Conclusion Recombinant human endostatin decreases eyclinD1 expression levels in AA synovial tissues, which may be one of the molecular mechanisms of the inhibitory effect of rhEndostatin on AA FLS proliferation.