中文摘要：

为了探讨菌根促生细菌荧光假单胞菌（Pseudomonas fluorescens）与菌根真菌的互作关系,本实验从油松菌根上分离得到36株在紫外灯下产荧光的细菌菌株,以荧光假单胞菌9702作为标准菌株,对分离菌株进行显微观察、生物学鉴定和16S rDNA序列分析,结果确定HDY-8、HDY-9、HDY-20、HDY-35共4株菌株为荧光假单胞菌,并分别命名为P.fluorescensHDY-8、P.fluorescensHDY-9、P.fluorescens HDY-20、P.fluorescens HDY-35。用这4株细菌菌株分别与外生菌根真菌（ECMF）粘盖牛肝菌（Suillus bovinus）、褐环粘盖牛肝菌（Suillus luteus）和褐黄牛肝菌（Boletus luridus）进行纯培养互作研究。结果表明,只有P.fluorescensHDY-20对3种外生菌根真菌有不同程度的促生作用,并对S.luteus促进效果最好,S.bovinus次之,B.luridus最差;P.fluorescensHDY-20促进S.bovinus、S.luteus和B.luridus菌丝生长的最佳浓度分别为2.4...

英文摘要：

To understand the regulating effect of nano-meter materils on the growth and development of forests,we examined the effects of 50～2 000 mg/L nano-meter TiO2 on the seed germination,seedling growth and physiological function of Pinus tabulaeformis.The results were as follows.Different concentrations of nano-meter TiO2 could all increase the seed germination,seedling growth and enhance physiological function to some degree,and the positive effects increased fast and then slowly with the rise of treatment concentrations.The 1 000 mg/L treatment had the best effect in increasing seed germination rate,germination index,germination energy,vigor index,seedling height and fresh weight and reducing mildewing rate,which were increased by 28.00%,18.00%,12.36%,126.95%,19.57%,36.89% and decreased by 15.33% respectively compared with the contrast.The 500 mg/L treatment had the best effect in increasing root length,root system activity,protein content,SOD and POD activities and decreasing the relative cell membrane permeability and malondialdehyde content,which were increased by 111.91%,53.85%,16.32%,170.93%,141.94% and decreased by 45.18% and 51.27% respectively compared with that of the contrast.The statistical analysis results showed that there were significant difference of all above indexes between optimal concentration treatment and contrast.It proved that proper concentration of nano-meter TiO2 could not only increase seed germination and seedling growth,but also ultimately improve physiological function and resistance to adversities of P.tabulaeformis＇s.