为筛选有效的植物杀菌成分,采用菌丝生长法,测定了香茅油、薰衣草油、菊花油、月桂油、柠檬油、广藿香油、肉桂油、天竺葵油、迷迭香油、茶树油、薄荷油对6种植物病原真菌的抑菌活性。发现在2 g/L的浓度下,上述11种精油对6种供试病菌均有明显的抑制作用,其中香茅油、肉桂油、天竺葵油、月桂油、茶树油和薄荷油对6种病原真菌的抑制率均为100%。剂量效应试验表明,肉桂油对灰葡萄孢(Botrytis cinerea)和禾谷镰孢菌(Fusariumgraminearum)的EC50值分别为29.05μg/mL和42.96μg/mL,而天竺葵油对灰葡萄孢(Botrytis cinerea)和禾谷镰孢菌(Fusarium graminearum)的EC50值分别为34.02μg/mL和68.48μg/mL。
Increasing concern over environmental and food safety caused by organic synthetic pesticides has highlighted the need for alternative products. Pesticidal activity screening from plants could lead to the discovery of new pest control agents. Antifungal activities of eleven plant essential oils were screened against six plant pathogenic fungi by mycelia growth methods. Results indicated that all tested essential oils possessed obvious antifungal activities against the six fungi at a concentration of 2 g/L, especially the antifungal activities of citronella, cinnamon, geranium, lavender, tea tree, and mint oils, which exhibited 100% inhibition against all tested plant pathogenic fungi. The EC50 values of cinnamon oil against Botrytis cinerea and Fusarium graminearum were 29. 05 μg/mL and 42. 96 μg/mL, respectively, and of geranium oil were 34.02 g/mL and 68.48 μg/mL, respectively.