为了促进草莓炭疽病菌在人工培养基上快速、大量地产生分生孢子,本研究在PDA培养基中添加不同量的酵母提取物,并结合涂断气生菌丝的方法,测定了不同处理对草莓炭疽病菌分生孢子产生的影响。结果发现,在PDA培养基中添加0.1%酵母提取物最适合草莓炭疽病菌(Colletotrichum theobromicola)的产孢。将培养3d菌落上的气生菌丝涂断,2d后可产生大量分生孢子。因此,PDA培养基中添加0.1%酵母提取物,结合菌丝涂断的方法,可显著提高草莓炭疽病菌的产孢量。该方法所用营养成分简单,用时短,操作简便。
In order to induce Colletotrichum pathogen from strawberry to produce a large number of conidia on media in a short time,the effects of two methods,i.e.,adding different contents of yeast extract(YE)to PDA medium and erasing the air mycelia on PDA,on sporulation of C.theobromicola were determined.The results indicated that PDA+0.1% YE was the optimal medium for sporulation of C.theobromicola.When the aerial mycelia growing 3 days on PDA medium were erased and continuously cultured for 2 days,the sporulation of the pathogen could be increased significantly.Therefore,using PDA added with0.1% YE and combined with erasing aerial mycelia is a convenient method for inducing massive sporulation of C.theobromicola.