采用叶盘漂浮法室内测定拮抗性多粘类芽孢杆菌PB-2对葡萄霜霉病的防治效果,结果表明,PB-2含菌量为108 cfu/mL的发酵液和过滤除菌的无菌发酵滤液对葡萄霜霉病菌均具有较好的抑制作用,抑制率分别达到了77.21%和60.24%.制备了PB-2可湿性粉剂,田间试验表明,PB-2可湿性粉剂稀释50倍和100倍液对葡萄霜霉病1周后相对防效分别为46.91%和36.99%,2周后分别为46.44%和42.10%.该研究表明PB-2可湿性粉剂对葡萄霜霉病具有良好的田间应用前景.
We employed floating leaf disc test to determine the control effects of antagonistic Paenibacillus polymyxa PB-2 against grape downy mildew.Results show that when PB-2 is 108 cfu/mL,the fermentation broth and aseptic fermentation filtrate both have better inhibitory effects on grape downy mildew.Their inhibitory rates are 77.21 % and 60.24%.We also prepared wettable powder (WP) of PB-2.Field tests indicate that the relative control effects of 50 and 100 times dilution PB-2 WP against grape downy mildew are 46.91 % and 36.99% after 1 week and 46.44% and 42.10% after 2 weeks.This demonstrates that PB-2 WP has better field application prospect for controlling grape downy mildew.