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蓝莓多酚的萃取及抗氧化活性研究
  • ISSN号:1671-1513
  • 期刊名称:北京工商大学学报(自然科学版)
  • 时间:2012.7.25
  • 页码:37-40
  • 分类:TS225.2[轻工技术与工程—粮食、油脂及植物蛋白工程;轻工技术与工程—食品科学与工程]
  • 作者机构:[1]山东省农业科学院农产品研究所,山东济南250100
  • 相关基金:国家自然科学基金青年基金资助项目(31101270)
  • 相关项目:蓝莓中可萃取与不可萃取多酚对巨噬细胞抗炎作用及差异性机理研究
中文摘要:

研究了不同萃取溶剂及pH值对蓝莓中可萃取多酚(EPP)和不可萃取多酚(NEPP)萃取率的影响,并用DPPH法研究了这两类多酚的抗氧化活性.结果表明,加入甲醇、乙醇、丙酮等有机溶剂可明显提高EPP的萃取率,其中以50%乙醇的萃取率最高.采用硫酸水解法分离制备NEPP,结果表明,蓝莓的NEPP含量远远高于EPP.EPP的抗氧化活性与其含量呈正相关性.萃取溶液pH值对EPP萃取率具有明显的影响,pH值越低,可萃取率越高.

英文摘要:

The effect of solvent and pH on the contents of extractable polyphenols (EPP) and non-ex- tractable polyphenols (NEPP) of blueberries was investigated in this paper. The antioxidant activity of polyphenols was also determined by DPPH system. The results showed that the solvent containing metha- nol, ethanol and acetone can enhance the EPP contents, especially 500/0 ethanol. The NEPP contents i- solated by the hydrolysis of sulfuric acid were much higher than that of EPP. There is a good correlation between the antioxidant activity and the EPP content. The yield of EPP increased with the decreasing of pH.

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