中文摘要：

雷电μs脉冲电磁场的危害越来越严重,却鲜有人研究此μs脉冲电磁场对人的影响。为研究μs脉冲磁场的生物电磁效应机制以及对人体健康的影响,使用人卵巢癌细胞株SKOV3作为实验对象,采用实验室自制的μs脉冲磁场发生器产生不同磁感应强度的μs脉冲磁场（磁感应强度为1~200 m T,上升时间为800 ns,脉冲宽度为8.4μs,频率为8 Hz）处理细胞3 d时间,每天间隔12 h时间处理1次,每次处理时间为1 h。处理结束后24 h时间分别使用噻唑蓝（MTT）和流式细胞术（flow cytometry method,FCM）来检测细胞的增殖抑制率和凋亡率。结果显示：增殖抑制率与脉冲磁场的磁感应强度不呈现趋势变化关系,且增殖抑制率均低于5%,μs脉冲磁场对SKOV3细胞的增殖抑制率不具有统计学意义（概率P〉0.05）;肿瘤细胞的凋亡率与脉冲磁场的磁感应强度也不呈现趋势变化关系,且凋亡率均低于12%,脉冲磁场对SKOV3细胞的凋亡也不具有统计学意义（概率P〉0.05）。研究结果揭示：不同磁感应强度（1~200 m T）的μs脉冲磁场对卵巢癌细胞SKOV3无显著影响。

英文摘要：

The damage of lightning pulsed electromagnetic fields becomes more and more serious, but few people study the impact of this microsecond pulsed electromagnetic field on people. In order to study the bioelectromagnetic mechanism of microsecond pulsed magnetic field and its effects on human health, human ovarian cancer cell line SKOV3 was used as subjects. A magnetic field pulse generator was adopted to generate a pulse magnetic field of different magnetic induction intensity （magnetic induction intensity of 1-200 naT, rise time of 800 ns, the pulse width of 8.4 gs, a frequency of 8 Hz） to treat cells 3 days, 12 hours interval per treatment, 1 hour each time. The MTT assay and FCM were used to detect the proliferation inhibition and apoptosis rate 24 hours after the end of treatment. The results show that the proliferation inhibition rate did not show the trend of change with magnetic induction intensity of pulse magnetic field, and the values of proliferation inhibition rate were all less than 5%. Microsecond pulse magnetic field had no significant effects on cell proliferation inhibition rate of SKOV3 （P〉0.05）; tumor cells apoptosis rate did not show the trend of change with magnetic induction intensity of pulse magnetic field, and the values were all lower than 12%. Microsecond pulse magnetic field had no significant effect on apoptosis of SKOV3 cells （P〉0.05）. The results reveal that different intensities of magnetic induction （1-200 roT） of microsecond pulsed magnetic field have no significant effects on ovarian cancer SKOV3.