中文摘要：

目的观察中药复方益肾达络饮干预实验性自身免疫性脑脊髓炎（EAE）的疗效和作用机制。方法采用荧光定量PCR测定免疫后不同时间点EAE小鼠中枢神经组织中CD4 mRNA表达的变化。结果中药复方益肾达络饮可以显著改善EAE模型小鼠神经功能评分。与模型组相比，激素组、中药组在免疫后14、24、40d CD4 mRNA在EAE小鼠大脑中表达水平明显降低，而在脊髓中仅在免疫后第14日显著降低。结论免疫炎性细胞CD4＋T细胞是实验性自身免疫性脑脊髓炎的潜在调节细胞：中药复方益肾达络饮能有效改善EAE小鼠神经功能损伤，其干预EAE的作用机制与中枢神经系统免疫炎性细胞的调节密切相关。

英文摘要：

Objective： To explore the efficiency and the mechanism of Yishendaluo Decoction to experimental autoimmune encephalomyelitis. Methods： RT-PCR was used to quantify the gene expression levels of expression of CD4 mRNA in the central nervous system at different time points. Results： Yishendaluo Decoction treatment of EAE could improve the neurological function score.Compared with model group,CD4 mRNA levels in the cere- brum were significantly.decreased at different time points of EAE including 14,24 and 40 days after immunization in hormone-treated group and Decoction-treated group ,but CD4 mRNA levels in the spinal cord of EAE were sig- nificantly decreased only at 14 days after immunization. Conclusions： CD4 T cells are potential mediators of EAE. Treatment with Yishendaluo Decoction can significantly improve the neurological function score. The therapeutic mechanism of Yishendaluo Decoction is to mediate the expression of immune/inflammatory cell.