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中文摘要：
在0．01mol·L-1。Hepes，0．15mol·L-1～NaCl，pH7．4及室温条件下，通过荧光光谱、圆二色光谱和紫外差光谱的方法研究了sm3＋与八肋游仆虫中心蛋白c端半分子（C-terminal domain of Euplotes octocarinatus eentfin，C-EoCen）III，IV结合位点的结合能力及结合后对蛋白质构象的影响。结果表明，Sm3＋与C-EoCen结合后，蛋白质从关闭式构象转变为开放式构象，蛋白的疏水性结构域外露程度增强，同时蛋白的α-螺旋含量明显增大；Sm3＋与C-EoCen的III，IV结合位点的条件稳定常数分别为：lgkⅢ=6．23±0．39，lgkIv=6．81±O．51。
英文摘要：
The binding of Sm3＋ with the C-terminal domain of Euplotes octocarinatus centrin （ C-EoCen ） were investigated by fluorescence spectra, circular di- chroism spectroscopy and difference UV-Vis spectra in 0.01 mol-L-I Hepes, 0.15 mol·L-1 NaC1 at pH 7.4 and room temperature. The Sm3 ＋ binding with C-Eo- Cen resulted in the conformation change of C-EoCen from closed state to open state and was accompanied by enhancement of the exposed hydrophobic surfaces. In addition, the cx-helix content of C-EoCen was in- creased by virtue of addition of Sm3 ＋. By introducing competitive ligand xylenol orange, the conditional binding constants of site HI and IV on C-EoCen with Sm3 ＋ were calculated to be lgKⅢ = 6.23 ± 0.39 and lgKⅣ =6.81 ±O. 51, respectively.
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