欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
用粒径15nm的纳米金标记单克隆羊抗人甲胎蛋白（GAFP），制备了甲胎蛋白（AFP）的免疫纳米金探针（AuGAFP）．纳米金及AuGAFP均对葡萄糖还原铜（Ⅱ）生成Cu2O微粒这一慢反应具有较强的催化作用，Cu2O微粒在620nm处产生1个较强的共振散射峰．将AFP—AuGAFP免疫反应与离心分离技术结合，建立了超痕量AFP的免疫纳米金催化-Cu2O微粒共振散射光谱新方法．随着AFP浓度的增大，AFP-AuGAFP免疫复合物微粒增多，离心液中AuGAFP浓度降低，620nm处的共振散射光强度I620nm线性降低，其降低值△IRS与AFP质量浓度P（AFP）在0．10～16．0．g／mL范围内呈现良好的线性关系，其回归方程为△IRS=4．27P（AFP）＋1．28，检出限为0．05ng／mL．本方法所用试剂易得，反应易控制，灵敏度高，选择性好，用于定量分析人血清中的AFP，结果令人满意．
英文摘要：
15 nm-nanogold was used to label monoclone goat antihuman α-fetoprotein antibody（GAFP） to obtain immunonanogold probe（AuGAFP） for α-fetoprotein （AFP）. Both nanogold and the probe have catalytic effect on the slow Cu20 particle reaction between Fehling reagent and glucose that exhibit a resonance scattering peak at 620 nm. Combining AFP-AuGAFP immunoreaction with centrifugation technique, a highly sensitive immunonanogold catalytic-Cu20 particle resonance scattering spectral assay for AFP was proposed. With addition of AFP, the AFP-AuGAFP immunocomplex increased, the excess probe in the supematant decreased, and the resonance scattering intensity at 620 nm decreased linearly. The decreased intensity AIRs was linear to AFP eoneentration[p（AFP）] in the range of 0. 10-16. 0 ng/mL, with a regression equation of AIRs = 4. 27p（AFP） ＋ 1.28, and a detection limit of O. 05 ng/mL. This method was applied to the detection of AFP in sera, with high sensitivity and good selectivity, in addition to low-cost reagents and easy controlling reaction.
同期刊论文项目
同项目期刊论文
Catalytic resonance scattering spectral determination of ultratrace horseradish peroxidase using rho
Resonance scattering spectral detection of catalase activity using Au@Ag nanoparticle as probe and c
Highly selective resonance scattering detection of trace thrombin using aptamer-modified AuRe nanopr
A highly sensitive and selective resonance scattering spectral assay for potassium ion based on apta
Resonance scattering spectral detection of trace Pb2+ using aptamer-modified AuPd nanoalloy as probe
A novel and sensitive resonance scattering assay for detection of urea in serum coupled urease catal
Resonance scattering spectral detection of trace ATP based on label-free aptamer reaction and nanogo
A new and sensitive catalytic resonance scattering spectral assay for the detection of laccase using
A new and sensitive catalytic resonance scattering spectral assay for the detection of laccase activ
Resonance Scattering Detection of Trace Hg2+ Using Aptamer-modified AuPd Nanoalloy Probe as Catalyst
Catalysis of aptamer-modified AuPd nanoalloy probe and its application to resonance scattering detec
Resonance scattering spectral detection of trace Hg(II) using aptamer modified nanogold as probe and
Immunonanogold-catalytic Cu2O-enhanced assay for trace penicillin G with resonance scattering spectr
An immunonanogold resonance scattering-quenching probe for rapid and sensitive assay of microalbumin
Catalytic effect of nanogold on Cu(II)-N2H4 reaction and its application to resonance scattering imm
Nanogold catalysis-–based immunoresonance immunoresonance-scattering spectral assay for trace comple
Immunonanogold-catalytic resonance scattering spectral assay of trace human chorionic gonadotrophin.
A selective resonance scattering assay for immunoglobulin G using Cu(II)-ascorbic acid-immunonanogol
An immunonanogold resonance scattering spectral probe for rapid assay of human chorionic gonadotroph
A rapid and highly selective resonance scattering spectral assay for trace carcinoembryonic antigen.
A highly sensitive sesonance scattering spectral assay for IgG using Fehling reagent-glucose-immunon
Resonance scattering detection of trace microalbumin using immunonanogold probe as the catalyst of F
A new and selective and sensitive nanogold-labeled immunoresoance scattering spectral assay for trac
期刊信息
位置：