中文摘要：

目的：构建Nar1基因的酵母表达载体,并纯化His-Nar1融合蛋白,为进一步研究Nar1的相互作用蛋白及其功能奠定基础。方法：通过PCR扩增Nar1全长基因;通过定向克隆的方法将Nar1片段与载体pYES2/NTC连接;通过乙酸锂转化法把重组质粒转化入野生型酵母菌株INVSc1;再通过免疫沉淀的方法纯化His-Nar1融合蛋白;通过Western blot检测靶蛋白。结果：DNA测序验证穿梭表达质粒pYES2/NTC-Nar1构建成功,Western blot检测到融合蛋白His-Nar1的表达。结论：成功构建Nar1酵母过表达载体,并且可以有效表达融合蛋白His-Nar1。

英文摘要：

Objective To construct the yeast expression vector of Narl gene and purify the fusion protein of His-Nar1, so as to provide the basis for the further research of interaction protein of Narl and their function. Methods The coding sequence of Narl gene was amplified by PCR ,the fragment of Narl gene and expression vector pYES2/NTC were ligated by directional cloning. The recombinant plasmid pYES2/NTC-Nar1 was transformed into INVSc1 ,then the fusion protein His-Nar1 was purified by immunoprecipitate method and identified by Western blot. Results The shuttle plasmid pYES2/NTC-Nar1 was successfully constructed which was confirmed by DNA sequencing, and the expression of fusion protein His-Nar1 was detected by Western blot. Conclusion The recombinant plasmid pYES2/NTC-Nar1 can successfully express His-Nar1 protein in yeast INVSc1.