以朗德鹅为对象,采用实时荧光定量PCR法研究不同填饲周龄脂肪酸合成酶基因(FAs基因)表达的规律性。结果显示,FAS基因相对表达量(FAS与β-actin基因表达量的比值)在填饲前、填饲1周、填饲2周、填饲3周和填饲4周相对表达量逐渐增高,分别为0.035、0.128、0.253、0.876、1.009,说明肥肝组织中FAS基因表达丰度与填饲期呈显著正相关,FAS基因在鹅肥肝形成过程及体脂沉积过程中具有一定的调控作用,为促进鹅肝内脂肪沉积的主要调控基因。
Landes geese were used as the materials to explore the expression rule ofFAS mRNA in fatty liver through real-time fluorescence quantitative PCR method during overfeeding weeks. Fluorescence quantitative PCR results showed that the expression of FAS gene revealed an obvious increase during pre-feeding fold and pro-feeding 1 week, feeding 2 weeks, feeding 3 weeks and feeding 4 weeks, which were 0.035, 0.128, 0.253, 0.876 fold and 1.009 fold enhancement, respectively. A positive genetic correlation between FAS mRNA expression in fatty liver tissue and feeding weeks was observed. These results suggested that FAS gene had a certain readjustment function during the developing process of fatty liver in goose and the process of body fat deposition. Thus, FAS is a regulation gene to promote fat deposit inside liver of goose, which provides valuable technical parameters for future studies of fatty liver.