中文摘要：

当先锋 l 酷氨酸被一个复杂 biosynthetic 系统紧地调整，在 Saccharomyces cerevisiae 的 heterologous resveratrol 生产的新陈代谢的工程面对挑战。我们主要基因在在上游的小径指向在下游的 resveratrol 生产上调查他们的影响的 overexpressed。单个基因的 overexpression 和 DNA 指导集会的 multigene overexpression 影响 resveratrol 以及它的先锋 p-coumaric 酸的生产。最后，选择基因目标的合作导致 66.14 的最佳的 resveratrol 生产 ? 铬 ? 铬 ?

英文摘要：

Metabolic engineering of heterologous resver- atrol production in Saccharomyces cerevisiae faces challenges as the precursor L-tyrosine is stringently regulated by a complex biosynthetic system. We over- expressed the main gene targets in the upstream pathways to investigate their influences on the downstream resver- atrol production. Single-gene overexpression and DNA assembly-directed multigene overexpression affect the production of resveratrol as well as its precursor p-coumaric acid. Finally, the collaboration of selected gene targets leads to an optimal resveratrol production of 66.144-3.74 mg.L-1, 2.27 times higher than the initial production in YPD medium （4% glucose）. The newly discovered gene targets TRP1 expressing phosphoribosy- lanthranilate isomerase, AR03 expressing 3-deoxy-D- arabino-heptulosonate-7-phosphate synthase, and 4CL expressing 4-coumaryl-CoA ligase show notable positive impacts on resveratrol production in S. cerevisiae.