中文摘要：

Pyrroloquinoline quinone (PQQ ) 在细菌与脱氢酶在联合作为一个氧化还原作用余因子起一个重要作用。这些脱氢酶为生物工学工业在重要底层的氧化起关键作用，例如维生素 C 生产。当 PQQ 基因的生合成广泛地被学习了时， PQQ 运输机制仍然保持不清楚。此处，我们使用了定序在 Gluconobacter oxydans WSH-003 的工业紧张调查 pqqB overexpression 的效果的两二维的荧光差别胶化电气泳动双人脚踏车团 spectrometry 和 RNA。我们鉴别了 73 差别表示了蛋白质， 99 差别表示了基因，其一个多数与氧化减小有关并且由基因本体论分析搬运过程。我们也描述了对源于 pqqB overexpression 的增加的 PQQ 层次作出回应的几个通常认为的候选人受动器。而且，量的 PCR 被用来在生产紧张的不同 PQQ 之中验证五通常认为的 PQQ 运输基因，并且结果证明 ompW， B932_1930 和 B932_2186 是处于所有条件的 upregulated。当时，三基因在 G 是过去表示的。oxydans WSH-003 和 PQQ 生产被检测。结果显示出 B932_1930 (transporter ) 的那细胞外的 PQQ， overexpression 拉紧的 B932_2186 (ABC transporter permease ) 被 1.77 褶层和 1.67 褶层分别地提高。结果建议 PqqB， B932_1930 和 B932_2186 编码的蛋白质可能提高 PQQ 分泌物过程。

英文摘要：

Pyrroloquinoline quinone （PQQ） plays a sig- nificant role as a redox cofactor in combination with dehydrogenases in bacteria. These dehydrogenases play key roles in the oxidation of important substrates for the biotechnology industry, such as vitamin C production. While biosynthesis of PQQ genes has been widely studied, PQQ-transport mechanisms used both two-dimensional remain unclear. Herein, we fluorescence-difference gel electrophoresis tandem mass spectrometry and RNA sequencing to investigate the effects ofpqqB overexpres- sion in an industrial strain of Gluconobacter oxydans WSH-003. We have identified 73 differentially expressed proteins and 99 differentially expressed genes, a majority of which are related to oxidation-reduction and transport processes by gene ontology analysis. We also described several putative candidate effectors that responded to increased PQQ levels resulting from pqqB overexpression. Furthermore, quantitative PCR was used to verify five putative PQQ-transport genes among different PQQ producing strains, and the results showed that ompW, B932 1930 and B932_2186 were upregulated in all conditions. Then the three genes were over-expressed in G. oxydans WSH-003 and PQQ production were detected. The results showed that extracellular PQQ of B932_1930 （a transporter） and B932_2186 （an ABC transporter perrnease） overexpression strains were enhanced by 1.77- fold and 1.67-fold, respectively. The results suggest that the proteins encoded by PqqB, B932_1930 and B932 2186 might enhance the PQQ secretion process.