中文摘要：

【目的】研究青霉素V生产过程中—Ca∽（2＋）信号转导途径参与产黄青霉菌对外源侧链前体苯氧乙酸的应答机制。【方法】考察4种不同机制的Ca∽（2＋）信号干扰剂[利心平、乙二醇双（2-氨基乙基醚）四乙酸、苏拉明和硫酸新霉素]对青霉素V产量和产黄青霉菌生物量的影响。运用Fluo-3/AM荧光染料对细胞进行染色,通过荧光显微镜成像和酶标仪定量检测两种方法监测胞内Ca∽（2＋）浓度的变化。【结果】苯氧乙酸添加后胞内Ca∽（2＋）相对含量高于对照组49.86%,而1 mmol/L磷酸酯酶C底物抑制剂硫酸新霉素的添加使得胞内Ca∽（2＋）相对含量降低了53.31%,同时青霉素V产量降低78.71%,表明产黄青霉菌可通过肌醇1,4,5-三磷酸信号途径调节胞内Ca∽（2＋）浓度来响应苯氧乙酸的胁迫。【结论】首次探究了Ca∽（2＋）信号转导途径在产黄青霉菌对苯氧乙酸应答中的作用,为丝状真菌中Ins（1,4,5）P3-Ca∽（2＋）信号转导途径的研究提供理论依据。

英文摘要：

[Objective] We studied calcium-associated signal responses of Penicillium chrysogenum to phenoxyacetic acid. [Metbods] The effects of different types of Ca2＋ interference agents （Nifedipine, EGTA, Suramin and Neomycin sulfate） for penicillin V production were studied. Intracellular Ca2＋ concentration was measured by Olympus System microscope and SpectraMax M2 Fluorescence Microplate Reader. [Results] Levels of intracellular Ca2＋ in P chrysogenum significantly increased （49.86% higher than the control） at 25 h after treatment with phenoxyacetic acid （POA）, whereas significantly reduced （53.31% lower than the control） after treatment with neomycin sulfate as an intracellular calcium inhibitor. Results indicate that cells regulated intracellular calcium level though Ins（1,4,5）P3 signaling pathways to response POA stress. [Conclusion] Understanding the effect of Ca2＋ signal transduction pathway how P. chrysogenum responses to POA stress provides guidance for industrial penicillin production.