中文摘要：

研究了以铁氰化钾为电子传递剂，亲水纳米二氧化硅为固定化酶的载体与高分子成膜材料掺杂制作的生物敏感膜修饰丝网印刷碳糊电极葡萄糖生物传感器的改进特性，并从机理上分析了形成这种优化的原因。实验采用柠檬酸作为缓冲液，在高分子成膜材料、铁氰化钾、稳定剂、葡萄糖氧化酶中掺杂均相处理后的纳米二氧化硅制备生物敏感膜，并制成腔体，将其与未经过纳米二氧化硅掺杂制备的生物传感器进行对比实验。实验证明：用掺杂纳米二氧化硅制作的生物敏感膜修饰的丝网印刷碳糊电极与未修饰电极相比，灵敏度提高了2．6倍，线性检测范围为1．1～33．3mmol／L，对测试范围内不同浓度的葡萄糖样本，相对标准偏差〈5％，重现性和稳定性良好，具有较高的研究价值和应用前景。

英文摘要：

In this study, K3Fe（CN6） acted as electron transport reagent, and glucose oxidase was immobilized in the multiple membrane matrix consisting of polymer and hydrophilic nano-SiO2 on screen-printed carbon paste electrodes. In this way a glucose biosensor modified with hydrophilic nano-SiO2 and polymer was completed. The experimental results showed that the sensitivity of carbon paste electrodes modified with nanoSiO2 was 2.6 times of unmodified, Measurable linear range is 1.1 - 33.3 mmol/L. To different concentration glucose, the RSDs were less than 5% with a better repeatability and stability. The experiment results turned out that the membrane of nano-SiO2 doping had a good hydration, nano-SiO2 could increase the quantity of immobilized enzyme, so the kinetic process of enzymatic reaction was improved greatly with potential applieation.