将1-(2-吡啶偶氮)-2-萘酚(PAN)和荧光试剂罗丹明B(RB)联合作为指示剂,再通过碱性催化的溶胶凝胶法将指示剂固定在与传像光纤相联结的自聚焦透镜端面上,成功制备了一种具有传像功能的荧光锌离子传感探头。与改进的金相显微镜和激光器相联接,可同时实现对锌离子浓度的检测和样品外貌的观测。此系统在锌离子浓度为0.1~1mmol.L-1时,存在较好的线性关系,且系统传像的分辨率为18.73lp.mm-1。此种具有传像功能的传感器在生物检测方面有较大的应用潜力。
A zinc ion fluorescence sensor with imaging was fabricated by using 1-(2-Pyridylazo)-2-naphthol and fluorescent reagent of rhodamine B together as the indication which was immobilized on an tip of gradient index lens by base catalyzed sol-gel method.Combined with improved metallographic microscope and laser,the measurement of zinc ion concentration and the view of sample morphology could be realized simultaneously by this system.The sensor has the linear range of zinc ion concentration from 0.1 to 1 mmol·L-1.Moreover,it has the capabilities of imaging with the whole system's resolution of 18.73 lp·mm-1.This sensor with the function of imaging has the potential applications in biosensing.