中文摘要：

通过对大豆吉林32未成熟胚表达谱的分析,利用RT-PCR技术从大豆中克隆了一个新的脯氨酸富集蛋白基因,命名为GmPRP。GmPRP的开放阅读框长396bp,其分子量13.79kD,具有131个氨基酸残基,等电点8.96,其DNA序列无内含子。GmPRP蛋白序列N端含有一段信号肽,中间为脯氨酸富集区,C端为半胱氨酸富集区。该蛋白与四季豆和木豆的PRP同源性最高,具有较近的亲缘关系。GmPRP的683bp启动子序列含有10种与逆境相关的顺式作用元件,分别为ABRE-like、G-box、W-box、GT-1、MYB、MYC、BIHD10s、DPBF、SEBF和WRKY。实时荧光定量PCR分析表明,该基因表达量在大豆的根和叶中最高,在茎和胚中其次,在花中最低,且受干旱、高盐、低温、机械伤害及SA（水杨酸）、ETH（乙烯）、ABA（脱落酸）、MeJA（茉莉酸甲酯）的诱导上调表达。

英文摘要：

Plant proline-rich proteins（PRPs） are putative cell wall proteins,which are usually associated with different abiotic and biotic stress conditions.A soybean mRNA sequence encoding a proline-rich protein（PRP） was cloned and designated as GmPRP from Jilin 32 immature embryo gene expression profiles using RT-PCR.The GmPRP consisted of an ORF with a length of 396 bp,and encoded 131 amino acids（13.79 kD） with an isoelectric point of 8.96.There was no intron in the DNA sequence of GmPRP.Except a repetitive proline-rich domain,GmPRP also contained a signal peptide in the N-terminal domain and a conserved eight cysteine motif in the C-terminal domain.The amino acid sequences of GmPRP,PvPRP and CcHyPRP shared high homology through phylogenetic analysis.The length of the promoter was 683 bp,containing several stress-induced elements：ABRE-like,G-box,W-box,GT-1,MYB,MYC,BIHD10s,DPBF,SEBF,and WRKY.Real-time quantitative PCR（qPCR） analysis revealed that GmPRP expressed highly in root and leaf and low in flower.qPCR was also performed to investigate the expression profiles of the GmPRP under different stresses such as drought,high salt,low temperature,wound,SA（salicylic acid）,ETH（ethane）,ABA（abscisic acid） and MeJA（methyl jasmonate）.Under these stresses GmPRP showed up-regulated expression patterns.These results revealed that GmPRP might be involved in multiple pathways of plants responding to the different environmental conditions.