中文摘要：

采用RT-PCR法克隆了大豆异黄酮生物合成途径中查尔酮合酶（Chalcone synthase,CHS）和查尔酮异构酶（Chalcone Isomerase,CHI）2个关键酶基因,并利用SOE-PCR技术成功将2个基因融合,将其均构建成原核表达载体,在大肠杆菌中进行了初步的表达研究,为进一步研究CHS和CHI 2种关键酶的功能奠定了基础。同时构建了真核表达载体,采用农杆菌转化法侵染大豆子叶节,获得了转基因抗性植株。

英文摘要：

Chalcone Synthase（CHS）and Chalcone Isomerase（CHI）are two key enzymes in soybean isoflavones biosynthesis pathway,the cDNAs of CHS and CHI were cloned using RT-PCR method and these two genes were fused using SOE-PCR,then constructed E.coli expression vectors to study the expression.The eukaryocyte expression vectors were also constructed and transferred into soybean cotyledonary nodes using Agrobacterium-mediated transformation,and then obtained the resistant transgenic plants.