中文摘要：

采用超高效液相色谱-四级杆飞行时间高分辨质谱联用技术（UHPLC-QTOF/MS）对RA264.7巨噬细胞甘油磷脂成分进行结构推断,运用修饰后的Bligh-Dyer法提取甘油磷脂,以流动相A：水（含10 mmol·L-（-1）乙酸铵和0.25%乙酸）,流动相B：乙腈-异丙醇（1∶1）（含10 mmol·L-（-1）乙酸铵和0.25%乙酸）进行梯度洗脱,在ESI正负离子模式下进行数据采集。本实验推断出82个甘油磷脂成分,其中包括57个磷脂酰胆碱（PCs）、21个磷脂酰乙醇胺（PEs）、3个磷脂酰甘油（PGs）和1个磷脂酰肌醇（PI）。本文建立的基于UHPLC-QTOF/MS液质联用技术的RAW264.7巨噬细胞甘油磷脂类成分分析方法快速、简便、可靠。

英文摘要：

An ultra performance liquid chromatography tandem quadrupole time-of-flight mass spectrometric method was developed for rapid analysis of glycerophospholipids in RAW264.7 macrophage. The modified Bligh-Dyer was applied to extract glycerophospholipids from RAW264.7 macrophage. The target compounds, detected by mass spectrometry in ESI＋ and ESI mode, were separated by gradient elution with mobile phase （A） water （containing 10 mmol·L-1 ammonium acetate and 0.25% acetic acid） and （B） acetonitrile/isopropanol （1 ： 1） （containing 10 mmol·L-1 ammonium acetate and 0.25% acetic acid）. A total of 82 glycerophospholipids in- cluding 57 phosphatidylcholines （PCs）, 21 phosphatidylethanolamines （PEs）, three phosphatidylglycerols （PGs） and one phosphatidylinositol （PI） were deduced. The UHPLC-QTOF/MS method is rapid, simple and credible for targeting analysis of glycerophospholipids of RAW264.7 macrophage.