位置：成果数据库 > 期刊 > 期刊详情页
RECQ5β解旋酶DNA退火特性的动力学研究
  • ISSN号：0023-074X
  • 期刊名称：《科学通报》
  • 时间：0
  • 分类：Q523[生物学—生物化学]
  • 作者机构：[1]西北农林科技大学生命科学学院,杨凌712100, [2]中国科学院物理研究所软物质物理实验室,北京凝聚态物理国家实验室,北京100190, [3]Institut Curie-Section de Recherche, Centre Universitaire, Batiment 110, F-91405 Orsay, France
  • 相关基金：国家自然科学基金重点项目（10834014）; 国家重点基础研究发展计划（2009CB930704）资助
作者： 丁秀艳[1,2], 徐雅楠[2], 李伟[2], 王鹏业[2], 奚绪光[1,3], 窦硕星[2]
关键词： 解旋酶, RECQ5β, DNA退火, 动力学, DNA结合
中文摘要：

RecQ家族解旋酶对维持基因组完整性起着关键作用.许多RecQ家族解旋酶不仅可以打开双链及其他更复杂的DNA结构,而且有意思的是,还具有DNA退火活性.本文通过对DNA退火动力学、平衡态下DNA结合以及与单链DNA解离动力学的一系列测量,系统研究了RECQ5β的DNA退火特性.结果显示,在本研究的反应缓冲液条件下,在没有或存在不同的磷酸核苷因子（AMPPNP,ATPS或ADP）的情况下,当酶分子覆盖约40%~50%DNA单链时,RECQ5β催化的DNA退火效率最高.通过对RECQ5β1~662的比较研究证实,RECQ5β的C末端区域对该酶高的DNA退火活性是必不可少的.本文结果将有助于更好地理解RecQ家族解旋酶催化DNA退火的机理.

同期刊论文项目
利用单分子和荧光方法研究生物大分子的动力学
期刊论文 81
同项目期刊论文
Partition function loop series for a general graphical model: free-energy corrections and message-pa
Selectivity and temperature dependence of phase and phase transition in diblock copolymer solution
原子力显微镜观测生物大分子图像的一种处理方法
Kinetic study of the DNA annealing properties of RECQ5 beta helicase
Studying the interaction between gyrase and DNA using magnetic tweezers
Circadian KaiC Phosphorylation: A Multi-Layer Network
EFFECT OF ELECTRON CORRELATION AND SOLVENT ON THE CONFORMATIONAL TRANSITION OF DNA
BLM蛋白解旋活性中心的DNA解旋动力学机理研究
Modelling of a DNA packaging motor
A Solution to a Single Molecular Experiment Problem
Investigation on Energetic Optimization Problems of Stochastic Thermodynamics with Iterative Dynamic
FoF1-ATPase, rotary motor and biosensor
Effects of CRAC Channel on Spatiotemporal Ca2+ Patterns in T Cells
用单分子技术研究抗癌药物顺铂对DNA结构的影响
Brownian dynamics simulation of the cross-talking effect among modified histones on conformations of
Linear response theory and transient fluctuation relations for diffusion processes: a backward point
Elastic response and length change of single DNA molecules induced by a combination of cisplatin and
Combined local search strategy for learning in networks of binary synapses
Approaching the ground states of the random maximum two-satisfiability problem by a greedy single-sp
Effect of Cisplatin on the Flexibility of Linear DNA
打开DNA双链的物理新方法-激光微流法
Effect of Laser Field and Mechanical Force on Deoxyribonucleic Acid Melting
Domain shapes in lipid monolayers studied as polar cholesteric liquid crystals
Icosahedral formation of nano-micro capsules: An energetic viewpoint
Study of the interaction of DNA and histones by spin-stretching and droplet evaporation
From Chemical Langevin Equations to Fokker-Planck Equation: Application of Hodge Decomposition and K
用单分子磁镊研究顺铂导致的DNA凝聚
Ground-state entropy of the random vertex-cover problem
Formation of DNA toroids inside confined droplets adsorbed on mica surfaces
Temperature dependence of circular DNA topological states
Spherical/gyroid phase diagram of the diblock copolymer in the median selective solvent
Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel
Forces-induced pinpoint denaturation of short DNA
Generalized integral fluctuation theorem for diffusion processes
The study of the elasticity of spider dragline silk with liquid crystal model
Dynamics-driven evolution to structural heterogeneity in complex networks
Mechanochemical Coupling of Kinesin Studied with a Neck-Linker Swing Model
A generalized integral fluctuation theorem for general jump processes
Study of axial strain-induced torsion of single-wall carbon nanotubes using the 2D continuum anharmo
Cytoplasmic Ca2+ Dynamics under the Interplay between the Different IP3R Gating Models and the Plasm
Multimeric BLM is dissociated upon ATP hydrolysis and functions as monomers in resolving DNA structu
Parallel Tempering Simulation on Generalized Canonical Ensemble
Diffusion-Limited Aggregation with Polygon Particles
Spherical tensor analysis of polar liquid crystals with biaxial and chiral molecules
Inactivation of A549 cancer cells by a helium-oxygen plasma needle
Counting solutions from finite samplings
Spontaneous Polygonization of Multiwalled Carbon Nanotubes: Perturbation Analysis (vol 51, 065101, 2
A big cluster model of the PER-TIM interval timer in drosophila cytoplasm for the circadian clock
Impact of DNA Twist Accumulation on Progressive Helical Wrapping of Torsionally Constrained DNA
Effects of Paclitaxel on EGFR Endocytic Trafficking Revealed Using Quantum Dot Tracking in Single Ce
Region Graph Partition Function Expansion and Approximate Free Energy Landscapes: Theory and Some Nu
Core Percolation on Complex Networks
果蝇昼夜生理节律之细胞质PER-TIM间隔定时器的大聚合物模型
利用磁镊研究促旋酶与DNA的结合
打开DNA双链的物理新方法——激光微流法
钙释放激活钙离子通道的发现及研究现状
利用轨迹自动识别方法研究单细胞内表皮生长因子受体的运输
Spermidine-induced two-dimensional DNA condensations on mica surfaces： A different pathway from condensations in solution
Kinetic study of the DNA annealing properties of RECQ5β helicase
Brownian dynamics simulation of the cross-talking effect among modified histones on conformations of nucleosomes
期刊信息
  • 《科学通报》
  • 北大核心期刊（2011版）
  • 主管单位:中国科学院
  • 主办单位:中国科学院
  • 主编：周光召
  • 地址：北京东黄城根北街16号
  • 邮编：100717
  • 邮箱：csb@scichina.org
  • 电话：010-64036120 64012686
  • 国际标准刊号：ISSN：0023-074X
  • 国内统一刊号：ISSN：11-1784/N
  • 邮发代号:80-213
  • 获奖情况:
  • 首届国家期刊奖,中国期刊方阵“双高”期刊,第三届中国出版政府奖
  • 国内外数据库收录:
  • 美国化学文摘（网络版）,美国数学评论（网络版）,美国工程索引,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊（2004版）,中国北大核心期刊（2008版）,中国北大核心期刊（2011版）,中国北大核心期刊（2014版）,中国北大核心期刊（2000版）
  • 被引量:81792