中文摘要：

目的：观察不同配比当归-川芎分别经3种制备方法（水提、50%醇提、先水提后醇提）所得药液的抗氧化活性,探讨当归-川芎（归芎）药对优化配伍比例。方法：Fenton法测定OH自由基清除率,MTT法测定H2O2损伤内皮细胞的活力,权重系数法将以上指标转化为单指标,并采用人工神经网络拟和归芎药对不同配比的抗氧化活性曲线。结果：归芎药对各组均显示抗氧化作用。3种不同提取方法中,水提取物对OH自由基清除率活性最强;醇提取物对OH自由基清除率活性最弱,但对内皮细胞保护活性最强。总抗氧化活性的优化比例,水提物为1：1,50%醇提物为1：1.5,先水提后醇提物为2：1。结论：当归-川芎不同配比与不同制法的抗氧化活性作用不同,且存在优化配伍比例。归芎水提液抗氧化活性的优比例与临床汤剂中常用的1：1配比相一致。

英文摘要：

AIM：To study the anti-oxidative effects of Radix Angelica and Rhizoma Ligusticum with different ratio extracted by water,ethanol,alcohol-water.METHODS：Hydroxyl radical generated in Fenton reaction system and oxidative insult in HUVEC induced by H2O2 were used in the present study.RESULTS：Water preparation exhibited the strongest scavenging activity against OH generated in a Fenton reaction system.Ethanol preparation had the weakest activity against OH,while exerted strongest activity on damaged HUVEC.Water preparation （1：1）,ethanol preparation （1：1.5） and alcohol-water preparation （2：1） were found to be the most potent.CONCLUSION：Radix Angelica and Rhizoma Ligusticum with different ratio exert markedly different anti-oxidative activities.The most reasonable ratio may appear 1：1 for water preparation and be consistent with clinical ratio in si-wu-tang,a famous TCM fomulae.