中文摘要：

目的：观察当归-川芎不同配比（1∶0,2∶1,1.5∶1,1∶1,1∶1.5,1∶2,0∶1）分别经3种制备方法（水提、醇提、先水提后醇提）提取后所得药液对血小板聚集和凝血酶原时间（PT）、凝血酶时间（TT）的影响,探讨当归-川芎（归芎）药对因配比组方的不同而产生功效的差异性。方法：家兔颈动脉取血,比浊法测定ADP诱导的血小板聚集率和PT,TT,并比较归芎药对对以上各指标的影响。结果：归芎药对各组均显示一定的抗血小板聚集和抗凝血作用（P〈0.01或P〈0.05）。归芎不同配比药对中1.5∶1对血小板聚集的作用弱于其他配比样品,对PT,TT的影响则是随着当归在药对中比例的增大而增强;3种不同的提取方法中,水提液对血小板聚集作用较弱,醇提液对PT影响较强而对TT影响较弱。结论：当归-川芎不同配比与不同制法所产生的抗血小板聚集和抗凝血作用不同,并可能与其功效差异相关,其内在的物质基础与相关机理值得深入研究。

英文摘要：

Objective：To observe the effect of Radix Angelica and Rhizoma Ligusticum with different proportions（1∶0,2∶1,1.5∶1,1∶1,1∶1.5,1∶2,0∶1） extracted by different methods（by water、by ethanol、firstly by alcohol then by water） on platelet aggregation and prothrombin time（PT）,thrombin time（TT）.Methods： Take blood from carotid artery of rabbit and measure the rate of platelet aggregation induced by ADP and PT,TT to compare their different effects.Results： Radix Angelica and Rhizoma Ligusticum with different proportions and preparation methods showed certain effects of anti-platelet aggregation and antithrombin（P0.01 or P0.05）.The effect on PT and TT enhanced along with the ratio of Radix Angelica gradually increases;The platelet aggregation effect was the weakest While the ratio of Danggui-Chuanxiong was 1.5∶1.Compared with other extracts,the solution extracted by water showed a weaker effect of platelet aggregation,while solution extracted by ethanol showed a stronger effect on PT than TT.Conclusion： The different traditional efficacy of Radix Angelica and Rhizoma Ligusticum with different proportions and preparation methods may be related with their different effects of anti-platelet aggregation and antithrombin,their intrinsic material basis and relevant mechanism are worthy of further study.