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人急性淋巴细胞白血病细胞容积激活性氯电流的研究

ISSN号：1000-4718
期刊名称：中国病理生理杂志
时间：2011.4.4
页码：677-681
分类：R733.71[医药卫生—肿瘤;医药卫生—临床医学]
作者机构：[1]暨南大学医学院,广东广州510632, [2]暨南大学药学院,广东广州510632, [3]南方医科大学南方医院血液科,广东广州510515
相关基金：国家自然科学基金资助项目（No.30771106;No.30870567;No.30871267;No.90913020;No.U0932004）; 广东省自然科学基金资助项目（No.07005974）
相关项目：基于GABA受体靶标的新型一叶萩型生物碱的发现及其构效关系和作用机制研究

关键词：氯通道, Molt4细胞系, 膜片钳术, Chloride channels, Molt4 cell line, Patch -clamp techniques

中文摘要：

目的：研究人急性淋巴细胞白血病细胞系（Molt4细胞）容积激活性氯电流。方法：采用膜片钳全细胞方式记录细胞外低渗液（160mOsmol/L）激活的Molt4细胞容积激活性氯电流,分析该电流的特性。结果：Molt4细胞在等渗溶液中背景电流较小且稳定,低渗溶液诱导激活容积激活性氯电流,该电流呈现较明显的外向优势,在钳制电压0mV～±120mV及脉冲波宽200ms条件下,没有观察到明显的电压依赖性失活和时间依赖性失活。该电流具有容积敏感性,可被细胞外高渗刺激所抑制。氯通道阻断剂他莫昔芬显著抑制容积激活性氯电流,对内向电流和外向电流的抑制作用无显著差异。结论：Molt4细胞膜存在容积敏感性氯通道;低渗刺激可激活该通道产生容积激活性氯电流;该通道对氯通道阻断剂他莫昔芬敏感。

英文摘要：

ATM：To characterize the chloride current activated by extracellular hypotonic stress in human acute lymphoblastic leukemia Molt4 cells.METHODS：The technique of whole-cell patch clamp recording was used to detect the volume-activated Cl~-current in Molt4 cells.The characteristics of the current were investigated.RESULTS：The background Cl~-current was weak and stable under isotonic condition.However,a large Cl~-current was induced by exposure of the cells to 47%hypotonic solution.The current showed a characteristic of outward rectification.No voltage-dependent inactivation and time-dependent inactivation were observed.The current was sensitive to the change of cell volume and was inhibited by extracellular hypertonic solution.Extracellular tamoxifen,which is one of the chloride channel blockers,significantly inhibited the current.The effects of tamoxifen were almost equal for both inward and outward currents （P0.05）.CONCLUSION：There are volume-activated chloride channels on the cell membrane of Molt4 cells. Exposure of the cells to a hypotonic solution activates the chloride channels and induces a volume-activated chloride current. The volume-activated Cl~-channels are sensitive to tamoxifen in Molt4 cells.

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