中文摘要：

目的：细胞黏附性能是评价神经组织工程基质材料的重要指标，拟对包含神经活性多肽片断的IKVAV多肽两亲性分子在体外进行自组装，并且检测其对PC12细胞黏附的影响。 方法：实验于2005-12／2006—03在华中科技大学附属协和医院骨科实验室完成。IKVAV多肽两亲性分子委托上海波泰生物科技公司合成，并用高效液相色谱仪和质谱仪进行纯化和分析。在IKVAV多肽两亲性分子中加入稀盐酸降低pH值引发其自组装，用不同浓度的IKVAV自组装材料包被培养板，培养PC12细胞，检测IKVAV对PC12细胞黏附的影响结果：①当IKVAV自组装材料的密度为0．58-15．0μg/cm^2 。时，能明显增强PC12细胞黏附，而且在一定的范围内随密度增加PC12细胞的黏附率升高。②在1h和3h各IKVAV密度组的细胞黏附率之间差异无显著性： 结论：IKVAV能够促进PC12细胞黏附，而且有一定的量效关系。同时，IKVAV促进PC12细胞黏附的作用迅速、稳定。

英文摘要：

AIM： The capability of cell adherence is an important indicator in evaluating the scaffolds of neural tissue engineering. To conduct in vitro self-assembly of neural bioactive polypeptide isoleucine-lysine-valinealanine-valine （IKVAV）, and detect its effect on attachment of PC12 cells. METHODS： The experiment was conducted in the Laboratory of Orthopaedics, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology from December 2005 to March 2006. IKVAV-PA was synthesized by Shanghai Botai Biological ＆ Technical Corporation, which was then purified and tested with high-performance liquid chromatography （HPLC） and mass spectrometry （MS） respectively. The diluted hydrochloric acid was added into IKVAV-PA solution to reduce the pH value and accordingly induce self-assembly. Cell culture plates were coated with different concentrations of IKVAV self-assembly peptide. PC 12 cells were cultured and added to IKVAV coated plates. The effect of IKVAV on the attachment of PC 12 cells were tested. RESULTS： ①IKVAV-PA could significantly promote the attachment of PC12 at the density ranged from 0.58 μg/cm^2 to 15.0 μg/cm^2. Moreover, within a certain range, the attachment rate of PC12 cells increased with the density increased. ②There was no significant differences in the attachment rate at the 1^st and 3^rd hour among each IKVAV groups. CONCLUSION： IKVAV can promote the attachment of PC12 cells, which is in a dose-effect relationship. Meanwhile, the effects of IKVAV on the attachment of PC12 cells is rapid and stable.