中文摘要：

目的：合成神经活性多肽IKVAV（isoleucine—lysine—valine—Marline—valine，异亮氨酸-赖氨酸-丙氨酸-缬氨酸）多肽两亲性分子，在体外进行自组装成为纳米纤维，并且初步检测其生物相容性。 方法：实验于2005-12／2006-03在华中科技大学附属协和医院骨科实验室完成。①IKVAV多肽两亲性分子委托上海波泰生物科技公司合成，并用高效液相色谱仪和质谱仪进行纯化和分析。②IKVAV纳米纤维凝胶自组装：分别取200μL 10，5和2．5g／L IKVAV两亲性分子（IKVAV Peptide—AmpHipHile，IKVAV—PA）溶液置入小玻璃瓶中，分别加入等体积的含有钙（200mg／L）、镁（97．67mg／L）二价阳离子的DMEM细胞培养基溶液，自组装成为5，2．5和1．25g／L的材料。通过HCl溶液和蒸汽使IKVAV-PA自组装。③透射电镜检测：用场发射能量过滤型透射电镜对自组装后材料进行检测。④生物相容性初步检测：培养L929细胞系，加入不同浓度的1KVAV自组装材料，各组IKVAV—PA的最终浓度分别为0，20，60，160，500mg／L，用CCK-8试剂盒检测IKVAV自组装材料对L929细胞存活和活性的影响。 结果：①IKVAV-PA合成后检测结果：成功合成了IKVAV多肽两亲性分子，相对分子质量为1351，纯度为98％。②IKVAV—PA自组装结果：加入含钙、镁二价阳离子的细胞培养基或者降低pH值均可引发其自组装。当凝胶中IKVAV—PA浓度为5g／L时，自组装形成的凝胶完整，有足够的支撑力维持凝胶的形状。但是当IKVAV—PA浓度为2．5g／L和1．25g／L时，自组装形成的凝胶不完整，只在局部形成凝胶颗粒。③透射电子显微镜检测结果：分子自组装形成直径为7．0～8．0nm的纳米纤维。④生物相容性和生物活性初步检测结果：当IKVAV自组装材料生物相容性鬼好，并且当IKVAV的浓度为60或160mg／L时，能增强L929细胞的活性。 结论：IKVAV多肽两亲性分子能

英文摘要：

AIM： To synthesize bioactive IKVAV （isoleucine-lysine-valine-alaninev-aline） Peptide-AmpHipHile （IKVAV-PA） and self-assemble it in vitro, so as to make a pilot study of its biocompatibility. METHODS： The experiment was conducted at the laboratory of Department of Orthopedics, Huazhong University of Science and Technology between December 2005 and March 2006. ①IKVAV-PA was synthesized by the Shanghai Botai Biological Technology Company, and purified and tested with high-performance liquid chromatography and mass spectrometry.②Self-assembly of IKVAV nanofiber gel： 200 μL, 10, 5 and 2.5 g/L IKVAV-PA solution were put into the small glass bottle, and DMEM cell culture media with divalent cation of calcium （Ca 200 mg/L） and magnesium （Mg 97.67 mg/L） was added to induce into 5, 2.5 and 1.25 g/L assembled material by HCl solution and steam. ③Transmission Electron Microscope （TEM） test： The assembled material was detected with TEM of field energy filtering type. ④Pilot study of biocompatibility： L929 cells were cultured and added different concentrations of IKVAV-PA assembled material with the teminal concentrations of 0, 20, 60, 160 and 500 mg/L. CCK-8 kit was used to test the effect of IKVAV assembled material on survival and bioactivity of L929 cells. RESULTS： ①Detection of IKVAV-PA： The IKVAV-PA was synthesized successfully. The relative molecular mass was 1 351 with the purity quotient of 98%. （②IKVAV-PA self-assembly： When cell adding culture media containing Ca^2＋ and Mg^2＋ or decreasing the pH value, IKVAV-PA could self-assemble to produce a gel containing 0.5 g/L peptide, which could maintain the appearance of gel. But IKVAV-PA with concentrations of 2.5 and 1.25 g/L, the self-assembled gel was not complete with only gel granules in local. ③TEM results： The assembled material was a kind of nanofiber with diameter of 7.0-8.0 nm. ④Pilot study of biocompatibility and bioactivity： IKVAV nanofibers with concentrations of 60 μg/mL or 160 μg/mL could