中文摘要：

目的观察自组装含IKVAV多肽凝胶材料与神经组织的生物相容性。方法肽溶于NaOH溶液中，调整pH至8．5，浓度为0．01g／ml，加DMEM／F12在盖玻片上触发多肽自组装为凝胶，透射电镜检测。取新生SD大鼠背根神经节及其游离细胞分为实验组与对照组，实验组中DRG及其游离细胞与凝胶材料联合培养。倒置相差显微镜观察DRG生长情况，免疫荧光染色结合镜下细胞计数检测凝胶材料的细胞毒性和黏附性。结果透射电镜显示：自组装凝胶为编织状纳米纤维。实验组中DRG轴突生长情况优于对照组。培养1、3d后，两组中活细胞比例差异元统计学意义。培养3、12 h 后，实验组中神经细胞黏附数显著高于对照组。结论含IKVAV多肽凝胶材料能促进DRG生长和神经细胞黏附，对细胞毒副作用小，可作为优良的神经组织工程支架材料。

英文摘要：

Objective To investigate the biological compatibility of gel self-organized from IKVAV-containing peptides with nerve tissue..Methods The peptide was dissolved in NaOH solution where pH was adjusted to 8.5 and the content of peptide was 0.01 g/ml. Peptide covered with coverslip was triggered to be self-organized into gel by the addition of DMEM/F12 ,which was detected by TEM. The dorsal root ganglion （DRG） explants were collected from newborn rats, and then the tissues were dispersed into individual cell suspension by primary isolated culture methods. The DRG and dissociated cells were divided into experimental （EG） and control group （CG）. In EG.DRG and cells were transplanted on the surface of gels. Growth of DRG neurons was observed by IPCM. The gel＇ s cytotoxicity and its effect on attachment of DRG neurons were detected by live/dead assay kit and immunofluorescence staining in combination with cell counting. Results TEM showed that the self-assembly gel was composed of network nanofibers. The growth of axons from DRG in EG was better than in CG. There was no significant.difference in percentage of living cells between EG and CG 1 and 7 days after culture. The number of attached DRG neurons in EG was greater than in CG 3 h and 6 h after culture. Conclusion The gel self-organized from IKVAV-containing peptides ,which can enhance the growth of DRG and attachment of DRG neurons, has low toxicity to cells and can be acted as a superordinary nerve tissue engineering scaffold.