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中文摘要：
神经肽是一类重要的生物物质，研究与发展其分离分析方法一直备受关注．反相-高效液相色谱、毛细管电泳和质谱是目前最为常用的肽分离分析技术．构成肽的20种氨基酸所含的各种功能团为肽的修饰提供了必要的反应位点，迄今，人们已发展了多种针对氨基酸、肽和蛋白质的标记物．其中，琥珀酰亚胺酯类试剂是伯胺和仲胺的优良标记试剂协引，其特点足可形成稳定的酰胺键，反应条件温和，且产率较高．
英文摘要：
Labeling analysis of three neuropeptides（ Met-enkephalin, Leu-enkephalin, and neurotensin） with 7-methoxycoumarin-3-carboxylic acid N-succinimidyl ester（MCSE） was investigated. The experimental conditions for labeling reaction and HPLC analysis of the neuropeptide derivatives were optimized. It was found that the labeling reatction could be achieved at 37℃ for one hour in H3BO：NaCl-Na2B4O7 buffer（pH = 8.3） with a labeling ratio of about 80%. The derivative mixture from the three peptides can be separated in a 40min linear gradient from 60% to 20% of solvent A（100 mmoL/L sodium acetate, pH =5.0） and from 40% to 80% of solvent B [ 60% （volume fraction） acetonitrile in water ]. The resulting labeled products were also confirmed by MALDI-TOF-MS analysis. Compared with the native neuropeptides, the resulting labeled products have a longer absorption and fluorescence wavelengths, which is beneficial to the effective elimination of background fluorescence interference from biological matrix.
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