自Ash和Knoll等创立表面等离子体共振成像(Surface Plasmon Resonance Imaging,SPRI)技术以来,使得生物分子点阵的无标记并行检测成为可能.迄今为止,SPRI主要采用单波长激发光源,检测到的是单色或黑白图像.虽然利用多波长激发光源进行SPRI研究具有许多优势(比如彩色图像鲜艳动人及信息含量高等),并在金属膜等研究中有所应用,但在分子类样品中的研究应用的报道则很少,原因可能在于没有商品仪器且彩色信号复杂、分析难度大等方面.为了探索其中的问题,
A method of color surface plasmon resonance imaging was developed and true color SPR images of solvents and BSA microdot arrays were recorded by using our laboratory-built device. The color signals, which were observed to be complementary to the resonance absorption of the incident light, depended on the feature of samples and the incident angle as expected. This new method looks to be a potential color analytical tool for high throughput detection of biochips.