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Fast and accurate measurement of diffusion coefficient by Taylor's dispersion analysis

ISSN号：1001-6538
期刊名称：科学通报(英文版)
时间：0
页码：3325-3332
语言：中文
分类：O316[理学—一般力学与力学基础;理学—力学]
作者机构：[1]Beijing National Laboratory of Molecular Sciences and Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China, [2]Graduate University of Chinese Academy of Sciences, Beijing 100049, China
相关基金：Supported by the National Natural Science Foundation of China （Grant Nos. 20435030 and 20628507）, Chinese Academy of Sciences and the Ministry of Science and Technology （Grant No. 2002CB713803）
相关项目：表面分析

关键词：扩散率, 非水样板, 离散分析, 毛细管电泳分析, diffusion coefficient, aqueous and nonaqueous samples, Taylor＇s dispersion analysis, capillary electrophoresis, fast and accurate measurement

中文摘要：

这调查瞄准了改进泰勒的表演“为在各种各样的溶剂的极小的溶质的散开系数的快、精确的测量的 s 分散分析。调查被监视溶质脉搏的紫外吸收山峰并且由山峰效率计算散开系数在一台毛状的电气泳动仪器上执行。与是的 L 本氨基丙酸主要测试溶质，一些关键因素后来包括特别毛状的尺寸，搬运人流动速度，注射体积和毛状的调节被揭示。当很快的移植和高样品集中引起了 o ve 休息 i 地席离子时，跟踪的山峰，大量样品注射和慢移植被发现低估散开系数。在 0.1 的中等流动速度—1 cm/s 与一 72.44 毛状嗯 I.D.x60 厘米(50 厘米有效) 在 25 deg C 维持了，水的 L 本氨基丙酸的散开系数被决定，给 7.02X10 ~ 的价值(有错误的 -6) cm~2/s <2% 并且相对标准差 < 0.2 %(n=3 ) 。方法被显示对象水的本氨基丙酸，丙酮，酚，甲苯和苯那样的各种各样的样品的测量适用，并且非水的苯(在乙醇或 1-butanol ) 。

英文摘要：

This investigation aimed at improving the performance of Taylor＇s dispersion analysis for the fast and accurate measurement of diffusion coefficient of a minute solute in various solvents. The investigation was carried out on a capillary electrophoresis instrument by monitoring the UV absorption peak of a solute pulse and calculating the diffusion coefficient by peak efficiency. With L-phenylalanine as a main testing solute, some key factors were afterward disclosed including especially the capillary size, carrier flow velocity, injection volume and capillary conditioning. Peak tailing, large volume of sample injection and slow migration were found to underestimate the diffusion coefficient while very fast migration and high sample concentration caused overestimation. At a moderate flow velocity of 0.1--1 cm/s with a capillary of 72.44μm I.D.x60 cm （50 cm effective） maintained at 25℃, the diffusion coefficient of aque- ous L-phenylalanine was determined, giving a value of 7.02×10^6 cm^2/s with error 〈2% and relative standard deviation 〈0.2 % （n=3）. The method was shown to be applicable to the measurement of various samples such as aqueous phenylalanine, acetone, phenol, toluene and benzene, and nonaqueous benzene （in ethanol or 1-butanol）.