中文摘要：

用KI、CsCl和丙烯酰胺对黑豆（Glycinemaxvar．）凝集素I（GMLI）进行内源荧光淬灭研究．天然黑豆凝集素Ⅰ在280nm波长激发下显示λmax为339nm的内源荧光发射光谱，表明凝集素荧光生色基团位于相对疏水的环境中；3种淬灭剂对GMLI的荧光淬灭属于动态淬灭机制．GML工分子的生色基团Trp残基对中性淬灭剂丙烯酰胺的可接近程度为100％，而对阴离子淬灭剂KI的可接近程度为81．6％，对阳离子淬灭剂CsCl的可接近程度为52．8％，表明GMLI中大部分Trp残基位于分子表面或近表面，只有小部分包埋于分子内部的疏水性环境中，并且Trp残基周围所处微环境带正电荷的比例比带负电荷的比例高．

英文摘要：

The effects of intrinsic fluorescence quenching on GML I were studied using KI, CsCl and ac- rylamide. Native GML I exhibited fluorescence emission maximum λmax at 339 nm upon excitation at 280 nm, indicating the presence of tryptophan in an relatively hydrophobic region. The results indicated that the mechanisms of fluorescence quenching on the GML I caused by three kinds of quenchers were the dynamic quenching. Fluorescence quenching showed that acrylamide, KI and CsCl were able to quench 100%.81.6%and 52.8% of the intrinsic fluorescence of GML I respectively, further supporting that tryptophan residues are mainly located on the surface or near surface of the GML I molecular and microenvironment surrounding the Trp residues with more positive charge than negatively charge.