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中文摘要：
为提高重组表达质粒pET32c-ZGA在大肠杆菌中的表达量，对表达重组风雨花凝集素基因工程茵的培养条件进行优化筛选，对影响蛋白质表达量和工程茵生长的因素如pH值、诱导温度、IPTG、接种量、诱导时间、补充碳源形式、诱导时机、无机离子等进行了探索．经过优化，重组融合目的蛋白占茵体总蛋白的61．8％，比优化前提高了13．4％．条件优化后，pET32c-ZGA工程茵经过放大培养，获得6g／L的湿茵体量，超声波破碎茵体，SDS-PAGE证实重组融合蛋白以包涵体形式表达．
英文摘要：
In order to inerease the expression of recombinant Zephyranthes grandiflora Agglutinin （ZGA）, the influences of culture conditions such as pH, temperature, inducting time, IPTG concentra- tion and carbon sources in growth medium, volume of medium, inoculum quantities, inoculum time and concentration of inorganic ions, i.e. Mgz＋ , Ca2＋ , NH＋ et al on expression of recombinant protein were studied. The results after optimization showed that the expression of recombinant fusion ZGA was 61.8% of the total bacteria protein, increasing about 13.4%. The selected clone was amplified in 2 Liter LB culture medium and induced by IPTG, and 6 g/L thalli per liter culture medium were obtained under optimized conditions. The thalli was selected and homogenized under high pressure. SDSPAGE demonstrated that the recombinant targeted proteins were mainlv indwelled in the inclusion body.
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