中文摘要：

运用同源克隆的方法设计简并引物，通过3’和5’RACE技术，从蜘蛛抱蛋（Aspidistraelatior Bulme）中克隆了编码甘露糖结合集素（Aspidistra elatior Bulme Lectin，AEL）的全长cDNA序列．其cDNA全长为1149bp，包含930bp的开放阅读框，编码309个氨基酸的前体蛋白．前体蛋白经翻译后剪切成分子量分别为12．31kD和12．26kD两个成熟蛋白亚基（AEL—DOM1，AEL—DOM2）．AEL—DOM1和AEL—DOM2分别具有两个和一个典型的甘露糖专一结合位点（QDNY）．

英文摘要：

The cDNA sequence of a mannose-binding lectin （AEL） of Aspidistra elatior Blume was cloned by the degenerate primers through RACE （rapid-amplification of cDNA ends） technology. The full-length cDNA was 1149 bp, and has a 930 bp open reading frame encoding a protein precursor with 309 amino acids. The protein precursor was processed to two subunits （AEL-DOM1, AEL- DOM2） with molecular weight 12.31 and 12.26 kD, respectively. There are two typical mannose-binding sites （QDNY） located in AEL-DOM1, and only one mannose-binding site located in AEL- DOM2.