以粒度小于0.088mm秸秆粉的酶解液为底物与热预处理活性污泥(其中TS%为6.77%,VS%为47.90%,COD为36.665g/L)进行厌氧发酵产氢实验,以累积产氢量和产氢速率为考察指标,研究不同热预处理(100℃水浴)时间、初始pH值、酶解液浓度、发酵温度对厌氧发酵产氢的影响,并利用修正的Gompertz方程对产氢过程进行回归分析,优化出最佳玉米秸秆酶解液厌氧发酵产氢的工艺参数。结果表明:活性污泥利用玉米秸秆酶解液进行厌氧发酵产氢时,当活性污泥热预处理时间为15min、初始pH值为5.0、玉米秸秆粉酶解液浓度为22.34mg/mL、发酵温度为40℃时,产氢效果最佳,此时最大累积产氢量达到653.98mL,最大产氢速率为15.89mL/h。
Anaerobic fermentation experiments were performed for bio-hydrogen production from enzymatic hydrolyzate of corn stover powder (its granularity less than 0.088 mm)using heat pretreated activated sludge (its TS% is 6.77%, VS% is 47.90%, COD is 36.665 g/L) as fermentation microorganism. Effects of heat pretreated time (water bath is 100 ℃), initial pH value, enzymatic hydrolyzate concentration, and fermentative temperature on yield and rate of hydrogen production were investigated. Modified Gompertz Model was adopted to predict the bio-hydrogen production process. The results of one-factor experiments showed that each factor had significant effect on bio-hydrogen production process, and the optimum parameters were chosen from the selected levels. All the results fit well to the Modified Gompertz Model. The highest cumulative hydrogen yield (653.98 mL) and hydrogen production rate (15.89 mL/h) were obtained with a heat pretreated time of 15 rain, initial pH value of 5.0, enzymatic hydrolyzate concentration of 22.34 mg/mL and fermentative temperature of 40 ℃.