中文摘要：

利用王草、象草、柳枝稷、紫花苜蓿这4种常见能源草的纤维素酶酶解液作为产氢底物,对其光合生物制氢性能进行了实验研究,以累积产氢量和产氢速率为考察指标,对比了不同类型能源草的产氢能力,并利用修正的Gompertz方程对产氢过程进行回归分析,验证了能源草作为光合制氢原料的技术可行性。结果表明,当产氢工艺条件为光合细菌接种量30%、温度30℃、光照度2 000 lx、发酵时间120 h时,紫花苜蓿产氢性能最好,王草次之,而象草和柳枝稷的产氢性能较差。王草、象草、柳枝稷和紫花苜蓿的累积产氢量分别为75.3、27.2、26.1和81.6 m L,最大产氢速率分别为7.83、3.5、4.33、14.75 m L/（h·L）。

英文摘要：

This paper mainly studied the photo-hydrogen production capability of using enzymatic hydrolysate to four different energy grasses,king grass,witchgrass,grassiness and alfalfa,as substrates.The cumulative hydrogen production and hydrogen production rate were taken as the indexes to contrast photo-hydrogen production capability of different energy grasses. Then modified Gompertz equation was used to perform regression analysis of hydrogen production process,and the technical feasibility using energy grasses as raw materials for photo-hydrogen production was verified. The results showed that among these four energy grasses,the hydrogen producing capability of alfalfa was the best followed closely by king grass,and the hydrogen producing capabilities of witchgrass and grassiness were feeblish,in the circumstances of 30% inoculum size,30℃,2 000 lx illuminance,120 h of fermentation time. The cumulative hydrogen production of king grass,witchgrass,grassiness and alfalfa was respectively 75. 3,27. 2,26. 1, and 81. 6 m L. The maximal hydrogen production rate was 7. 83,3. 5,4. 33, and14. 75 m L /（ h·L） respectively.