中文摘要：

文章以小麦秸秆为原料,研究了不同接种量对产气肠杆菌同步糖化发酵产氢的影响,以期寻求最佳的接种量条件。试验以累积产氢量、产氢速率等指标来分析产气肠杆菌利用小麦秸秆进行同步糖化发酵产氢的潜力及其可行性。结果表明：在以反应液体积为200 mL,底物为5 g小麦秸秆,酶负荷为150 mg·g~（-1）秸秆、初始pH值为6.5,温度为35℃的条件下,接种量为30%时产氢效果最好,此时的累积产气量达到737 mL,累积产氢量达到293mL,最大产氢速率为35.42 mL·h~（-1）L~（-1）。该实验研究为秸秆类生物质同步糖化厌氧暗发酵产氢的进一步研究奠定了基础并提供了科学参考。

英文摘要：

Wheat straw was taken as raw material, the effect of different inoculum size on the hydrogen production by enter- obacter aerogenes with simultaneous saccharification in dark fermentation, were studied in this paper, aiming at seeking the best inoculum size. Parameters of cumulative hydrogen production and its production rate were taken as indicators for analy- zing. The results showed that, under the condition of 200 mL of reaction liquid, 5 g of wheat straw substrate, 150 mg of enzyme per gram of substrate,initial pH value of 6.5, and temperature of 35℃, the best inoculum size was 30%, from which the cumulative hydrogen production was 293 mL, the maximum hydrogen production rate reached 35.42 mL h-1 L-1.