中文摘要：

以产气肠杆菌（Enterobacteraerogenes）为纯菌种，葡萄糖（G）为发酵底物，分别选取25、30、35、38℃的发酵温度，初始pH值为3．5、4．5、5．5、6．5的酸碱度，葡萄糖与蛋白酶消化物的质量比为2、3、6的碳氮比的实验条件进行厌氧发酵产氢工艺及其代谢调控途径的实验。实验结果表明，产气肠杆菌具有一定的耐酸性，耐酸范围为pH值4．5—7．0；不同工艺条件下产气肠杆菌发酵产氢的周期在68～156h之间。产气肠杆菌发酵产氢周期最短为68h的最优化发酵工艺条件为温度35℃、pH值6．5、碳氮质量比3，其氢气产率达261．5mL／g，氢气转化率为2．1mol／mol。

英文摘要：

Experiments were carried out to investigate anaerobic fermentation hydrogen production process and its metabolic regulation ways with taking Enterobacter aerogenesenes as pure strains and using glucose as substrate. The experiments were conducted to study at different temperatures （25℃ ,30℃, 35~C, 38℃ ） , initial pH values （3.5,4.5,5.5,6.5） , mass ratio of glucoses （G） and protease digestion （2,3, 6）. The results showed that Enterobacter aerogenesenes had a certain acid-resisting in the pH value range of 7.0 to 4.5. Moreover, fermentative hydrogen production cycles of Enterobacter aerogenes was very variable （between 68 h to 156 h） under different technical conditions. The optimum conditions of the shortest fermentative hydrogen production cycle of 68 h were as follows：the temperature was 35 ℃ , the pH value was 6.5, meanwhile, the carbon - nitrogen ratio was 3. Based on these conditions, hydrogen production rate and hydrogen conversion rate could reach to the value of 261.5 mL/g and 2.1 mol/mol.