中文摘要：

[摘要]目的观察艾灸对幽门螺杆菌胃炎模型大鼠胃黏膜组织形态学变化、血清IL-6、IL-8、TNF-α、Hp—IgG、CD3^＋、CD4^＋、CD8^＋的影响，探讨艾灸干预HP胃炎胃粘膜损伤的机制。方法40只SD大鼠随机分为4组，每组10只，即A组（空白组）、B组（Hp胃炎模型组）、C组（艾灸＋模型组）、D组（电针＋模型组）。除A组外其余各组大鼠均以NaHCO：消炎痛＋Hp灌胃造模。各组经相应的干预后，HE染色光镜下观察大鼠胃黏膜组织形态学变化，Elisa法测定血清中IL-6、IL-8、TNF—α、Hp—IgG的含量，流式细胞术测CD3^＋、CD4^＋、CD8^＋、CD4^＋，CD8^＋值。结果与A组相比，B组大鼠血清IL-6、IL-8、TNF—α、Hp—IgG、CD8^＋明显增高（P〈0．01），CD3^＋、CD4^＋、CD4^＋／CD8^＋明显降低（P〈0．01）。与B组相比，C组血清IL-8、TNF-α、IL-6含量、Hp—IgG、CD8^＋明显降低（P〈0．05或0．01），CD3^＋、CD4^＋、CD4^＋／CD8^＋明显升高（P〈0．05），D组各指标无明显变化（P〉0．05）。与D组相比。C组IL-6、IL-8、Hp—IgG明显降低（P〈0．05），CD4^＋/CD8^＋明显升高（P〈0．05）。结论艾灸防治幽门螺杆菌胃炎的作用可能通过两种途径实现。抑制促炎细胞因子IL-6、IL-8、TNF-α的分泌，促进免疫因子CD3^＋、CD4^＋产生，抑制CD8^＋分泌，且艾灸对胃黏膜组织保护作用比电针更明显。

英文摘要：

Objective To explore the effects of moxibustion on the morphologic changes of gastric mucosa tissues and expression of serum IL-6, IL-8, TNF-α, Hp-IgG, CD3^＋, CD4^＋, CD8^＋, CD4^＋/CD8^＋in Helicobacter pylori（Hp）-associated gastritis model rats,to reveal the mechanisms underlying the protective effect of moxibustion against gastric inflammatory injury. Methods 40 healthy SD rats were randomly divided into 4 groups：the group A（blank group）, group B（Hp model group）, group C（moxibustion model groups） and group D（electro-acupuncture model group）, 10 rats in each group.The rats except for the group A were modeled by oral gavage with Na HCO3＋indomethacin＋Hp. After the intervention,the morphologic changes of gastric mucosa tissues in rats were observed by HE staining. The contents of serum IL-6, IL-8,TNF-α, Hp-IgG were detected by ELISA, and Contents of CD3^＋, CD4^＋, CD8^＋and CD4^＋/CD8^＋were detected by flow cytometry. Results Compared with group A, the expression of serum IL-6, IL-8, TNF-α, Hp-IgG, CD8^＋in group B were significantly increased（P〈0.01）, and contents of CD3^＋, CD4^＋and CD4^＋/CD8^＋were decreased obviously（P〈0.01）. Compared with group B, the expression of serum IL-6, IL-8, TNF-α, Hp-IgG, CD8^＋in group C were significantly decreased（P〈0.05 or0.01）, contents of CD3^＋（P〈0.05）、CD4^＋（P〈0.05） and CD4^＋/CD8^＋were increased significantly（P〈0.05）, no significant difference in group D（P〈0.05）. Compared with group D, the expression of IL-6, IL-8, Hp-IgG in group C were significantly decreased（P〈0.05 or 0.01）, CD4^＋/CD8^＋increased significantly（P〈0.05）. Conclusion The mechanisms of moxibustion in treatment of H. p ylori-induced gastric mucosal inflammatory injury were probably through two pathways, Inhibition of proinflammatory cytokine IL-6, IL-8, TNF-αsecretion, and promoting immune factor CD3^＋、CD4^＋secretion to inhibit the secretion of CD8^＋. The protective effect of moxibustion o