在房间 apoptosis 和热吃惊蛋白质( HSP )的表情上与 Zusanli (圣 36 )和 Zhongwan ( RN 12 )的 acupoints 调查 moxibustion-acupoint 处理的效果的目的 60 ,在老鼠的 caspase ( Smac )的 HSP70 和秒 mitochondrial 使活跃之物尖锐胃的 mucosal 损害( AGML )当模特儿,并且探索位于胃的 mucosal 的保护下面的机制损害。24 只 Sprague Dawley 老鼠被划分成 3 个组,的方法挡住控制的组(组 A ) ,控制点组(组 B ) 和 acupoint 组(组 C ) , 8 为各个。在在组 B 和 C 的 8 天的艾灸处理以后,无水的乙醇的胃的 lavage 在所有三个组习惯于创造 AGML。Guth 方法被采用测量胃的 mucosal 损害的溃疡索引(UI ) , immunohistochemistry 过去常与 apoptosis 索引(AI ) 测量 apoptosis 并且检验 HSP60, HSP70 和 Smac 的表情。结果显著地在组 B 与组 A, UI 的表情, AI, Smac 和 HSP60 相比被提高(P < 0.05 或 P < 0.01 ) 。然而, HSP70 的表示没显示出明显的变化(P > 0.05 ) ;UI, HSP60 和 HSP70 的表情显著地在组 C 被提高(P < 0.01 ) 当那些 AI 和 Smac 变得显然压制了时(P < 0.01 ) 。与组 B 相比, UI, AI 和 Smac 的表情在组 C 显著地减少了(P < 0.01 ) 当那些显著地 HSP60 和 HSP70 增加了时(P < 0.01 ) ,并且 HSP60 和 HSP70 的表情是更加起来调整的(P < 0.01 ) 。艾灸处理能减轻胃的 mucosal 损害的结论由无水的乙醇引起了,导致在表示上 HSP60 和 HSP70,和下面调整 Smac 的表示,它能压制房间 apoptosis。
OBJECTIVE: To investigate the effect of moxibus- tion-acupoint treatment with acupoints of Zusanli (ST 36) and Zhongwan (RN 12) on cell apoptosis and the expressions of heat shock protein (HSP) 60, HSP70 and second mitochondrial activator of cas- pase (Smac) in rat models of acute gastric mucosal lesion (AGML), and explore the mechanisms under- lying protection of gastric mucosal lesion. METHODS: Twenty-four Sprague Dawley rats were divided into 3 groups, blank controlled group (group A), controlled-point group (group B) and acupoint group (group C), 8 for each. After 8-day moxibustion treatment in group B and C, gastric la- vage of anhydrous ethanol was used to created AGML in all three groups. The Guth method was employed to measure the ulcer index (UI) of gastric mucosal lesion and immunohistochemistry used to measure apoptosis with apoptosis index (AI) and ex- amine the expressions of HSP60, HSP70 and Smac.RESULTS: Compared with group A, the expressions of UI, AI, Smac and HSP60 were markedly elevated in group B (P〈0.05 or P〈0.01). However the expres- sion of HSP70 showed no obvious change (P〉0.05), the expressions of UI, HSP60 and HSP70 were mark- edly elevated in group C (P〈0.01) while those of AI and Smac became obviously suppressed (P〈0.01). Compared with group B, the expressions of UI, AI and Smac decreased significantly in group C (P〈 0.01) while those of HSP60 and HSP70 increased markedly (P〈0.01), and the expressions of HSP60 and HSP70 were considerably up-regulated (P〈 0.01). CONCLUSION: The moxibustion treatment could al- leviate the gastric mucosal lesion caused by anhy- drous ethanol, induce the over-expressions of HSP60 and HSP70, and down-regulate the expres- sion of Smac, which could suppress cell apoptosis.