中文摘要：

吉氏巴贝斯虫BgTRAP蛋白是一个重要的诊断抗原候选分子。为建立一种实用的犬吉氏巴贝斯虫血清学诊断方法，本研究选取BgTRAP C-末端跨膜区前，包含TSP功能区和抗原区的411个氨基酸的编码基因片段，重组表达了一个可溶性截短型BgTRAP抗原，解决了完整蛋白重组表达纯化的困难。免疫荧光试验表明，截短型抗原具有良好的抗原性。纯化的截短型抗原作为酶联免疫试验诊断抗原，可清晰地区分阴性及阳性犬血清，并与其他病原感染无交叉反应，具有良好的特异性。犬感染吉氏巴贝斯虫系列血清检测表明，该抗原可检测早期感染（4 d）和感染200 d以后的样本。结果提示，重组BgTRAP截短型抗原可作为一种诊断制剂检测犬吉氏巴贝斯虫抗体。

英文摘要：

The BgTRAP protein of Babesia gibsoni is an important candidate for the development of a diagnostic reagent for canine babesiosis. In order to develop a diagnostic method for practical use, the gene fragment encoding truncated BgTRAP that covered TSP region and antigenic area ahead of transmembrane region was cloned and expressed in E.coli in soluble form. The recombinant truncated BgTRAP protein showed strong antigenicity in indirect fluorescent-antibody test and ELISA. The ELISA method using truncated BgTRAP protein was able to differentiate B. gibsoni-infected dog serum and non-infected dog serum. In dogs experimentally infected with B. gibsoni, ELISA method also detected antibody response as early as 4 days, or as late as 200 days post infection. These results demonstrated that the recombinant truncated BgTRAP protein might be a useful diagnostic reagent for detection of B.gibsoni antibodies in dogs.