中文摘要：

目的调查我国广西壮族自治区某食蟹猴养殖场血液寄生原虫感染状况,为人体血液寄生原虫的防控提供科学依据。方法采集广西壮族自治区南宁市某食蟹猴养殖场猴血液样本993份,全部制作FTA卡样本,涂制薄血膜550份。将样本混合,以巢式PCR及普通PCR方法分别检测FTA卡保存的猴血样本中巴贝虫以及疟原虫。检测阳性组再分别进行单个样本检测,阳性样本对应的薄血膜进行姬姆萨染色后再镜检。结果经巢式PCR检测,田鼠巴贝虫检出阳性率为6.95%（69/993）;仅1例经PCR检出猪尾猴疟原虫阳性。22份PCR检测巴贝虫阳性的血液样本经染色镜检,共16份检出巴贝虫,其显微镜下观察到红细胞内有环状体,无疟色素。结论我国广西地区食蟹猴的田鼠巴贝虫感染率较高,其可能在传播中起保虫宿主的作用;在筛查田鼠巴贝虫低密度感染时,巢式PCR方法敏感性较高。

英文摘要：

Objective To investigate the infection situation of blood parasitic protozoa in farmed Macaca fascicularis in an animal breeding ground in Nanning,Guangxi Zhuang Autonomous Region,so as to provide the evidence for the prevention and control of human blood parasitic protozoa.Methods A total of 993 blood samples from farmed M.fascicularis were collected and stored on FTA cards.Among them,550 thin blood smears were made.Each 10 samples were mixed in groups,and then the Babesia spp.and Plasmodium spp.in the blood of M.fascicularis were detected by Nest-PCR and PCR,respectively.The positive groups were tested individually.The thin blood smears stained with Giemsa were examined microscopically when PCR reported the samples were positive.Results When detected by Nest-PCR,the positive rate of Babesia.microti was 6.95%（69/993）;only 1 positive sample with Plasmodium inui was detected by PCR.Among the 22 positive thin blood smears detected by PCR,16 were determined with B.microti by microscopic examinations,on which the ring forms could be observed in the erythrocytes,but no hemozoin.Conclusions The positive rate of B.microti in M.fascicularis in Guangxi Zhuang Autonomous Region is high,and the animal may play a role as a reservoir host in the transmission of B.microti.In the screening of B.microti with low infection density,Nest-PCR has a higher sensitivity.