中文摘要：

目的：研究西藏绿萝花提取物对α-葡萄糖苷酶的抑制及抗氧化作用。方法：将西藏绿萝花的70％乙醇提取物依次用石油醚、乙酸乙酯、正丁醇萃取，然后测定各部分对α-葡萄糖苷酶的抑制及抗氧化作用。结果：乙酸乙酯的萃取部分对α-葡萄糖苷酶的活性抑制较强，半数抑制浓度IC50为43．63μg／mL，比阿卡波糖抑制α-葡萄糖苷酶的IC50值250μg／mL低的多，抵制动力学表明为竞争性抑制，Ki值为21．49μg／mL；乙酸乙酯及正丁醇萃取部分具有较强抗氧化作用，0．42μg／mL的乙酸乙酯及正丁醇萃取部分对DPPH·自由基的清除率分别可达到88．5％、93．6％，与0．3mg／mL的抗坏血酸对DPPH·自由基的清除率96．3％相当；乙酸乙酯萃取部分对氧自由基亦有一定的清除作用，IC50为0．27mg／mL。结论：西藏绿萝花醇提取物的乙酸乙酯萃取部分有较强的α-葡萄糖苷酶抑制及抗氧化作用；正丁醇萃取部分有较强的抗氧化作用。

英文摘要：

Objective ： To study the inhibitive effect of extracts from Scindapsus aureus on α-glucosidase and its antioxidant activity. Methods： The 70% ethanol extracts was extracted by petroleum, ethyl acetate and n-butanol, and the inhibitory activity against α- glucosidases and antioxidative effects of each fraction were determined in vitro, and the inhibitory kinetics of ethyl acetate fraction was investigated. Results： The inhibitory activity of ethly acetate fraction against α-glucosidase was higher as its IC5o was 43.63 μg/mL The inhibition kinetics analyzed by Hnewaver-Burk plots showed that ethyl acetate fraction was a competitive type inhibitor, and the inhibition constants for free ennzyme （Ki） was determined to be 21.49μg/mL The antioxidative effects of ethly acetate and n-butanol fractions exhibited stronger than that of other fractions, the scavenging ability on DPPH · at the concentration of 0. 42 mg/mL were 88. 5% and 93.6%. Ethly acetate fraction showed scavenging ability on autioxidantor, and the ICs0 values was O. 27 mg/mL Conclusion： Ethyld acetate fraction of extract from Scindapsus aureus has potential inbibitroy activity against α-gtucosidase and antioxidant effects, and n-butanol fraction has great antioxidative effects.