目的探讨脑性瘫痪(CP)患儿与正常同龄儿童在微量元素、免疫功能方面是否存在差异,为CP患儿补充微量元素和免疫调节治疗提供依据。方法采用原子吸收光谱法检测全血样本中铜(Cu)、锌(Zn)、钙(Ca)、镁(Mg)、铁(Fe)5种元素含量;采用速率散射比浊法检测血浆中IgG、IgA、IgM、C3含量;使用BDFACSCalibur流式仪测定全血中CD3、CD4、CD8含量。结果 CP组Cu、Zn、Ca、Fe、CD3、CD8、IgG均低于健康对照组,差异有统计学意义(P〈0.05)。结论 CP患儿与正常同龄儿童相比存在微量元素缺乏、体液及细胞免疫功能紊乱,据此为CP患儿补充微量元素和调节免疫治疗提供了一定依据。
Objective To explore the differences of microelement and immune function between children with cerebral palsy(CP) and healthy children,in order to provide a basis for therapy.Methods Atomic absorption spectrometry was used to detect the contents of Cu,Zn,Ca,Mg and Fe in whole blood samples.Rate nephelometry was performed to detect plasma levels of IgG,IgA,IgM and C3.BD FACSCalibur flow instrument was used to detect contents of CD3,CD4 and CD8 in whole blood samples.Results Levels of Cu,Zn,Ca,Fe,CD3,CD8,IgG and IgA in CP group were lower than those in healthy control group(P〈0.05).Conclusion Compared with healthy children,Children with CP might be with deficiency of microelement and dysfunction of humoral and cellular immune.Results of this research could supply certain basis for supplement of micronutrient and immune therapy in children with CP.